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Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function
https://oist.repo.nii.ac.jp/records/1272
https://oist.repo.nii.ac.jp/records/1272d98023e8-d103-44e7-a82b-e9e8af19975e
名前 / ファイル | ライセンス | アクション |
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200214_NChemBiol_Tsunoyama (25.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-03-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Tsunoyama, Taka A.
× Tsunoyama, Taka A.× Watanabe, Yusuke× Goto, Junri× Naito, Kazuma× Kasai, Rinshi S.× Suzuki, Kenichi G. N.× Fujiwara, Takahiro K.× Kusumi, Akihiro |
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書誌情報 |
en : Nature Chemical Biology 巻 14, 号 5, p. 497-506, 発行日 2018-04-02 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Single-fluorescent-molecule imaging tracking (SMT) is becoming an important tool to study living cells. However, photobleaching and photoblinking (hereafter referred to as photobleaching/photoblinking) of the probe molecules strongly hamper SMT studies of living cells, making it difficult to observe in vivo molecular events and to evaluate their lifetimes (e.g., off rates). The methods used to suppress photobleaching/photoblinking in vitro are difficult to apply to living cells because of their toxicities. Here using 13 organic fluorophores we found that, by combining low concentrations of dissolved oxygen with a reducing-plus-oxidizing system, photobleaching/photoblinking could be strongly suppressed with only minor effects on cells, which enabled SMT for as long as 12,000 frames (~7 min at video rate, as compared to the general 10-s-order durations) with ~22-nm single-molecule localization precisions. SMT of integrins revealed that they underwent temporary (<80-s) immobilizations within the focal adhesion region, which were responsible for the mechanical linkage of the actin cytoskeleton to the extracellular matrix. | |||||
出版者 | ||||||
出版者 | Nature Research | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1552-4450 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1552-4469 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/29610485 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1038/s41589-018-0032-5 | |||||
権利 | ||||||
権利情報 | © 2018 Nature America Inc.,part of Springer Nature. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.nature.com/articles/s41589-018-0032-5#Abs1 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |