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Design of Mammalian ON-Riboswitches Based on Tandemly Fused Aptamer and Ribozyme
https://oist.repo.nii.ac.jp/records/1481
https://oist.repo.nii.ac.jp/records/1481ac25fb7a-0a6e-4ba8-b494-345b5d7a1879
名前 / ファイル | ライセンス | アクション |
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Mustafina-2020-Design of Mammalian ON-Riboswit (1.4 MB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-05-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Design of Mammalian ON-Riboswitches Based on Tandemly Fused Aptamer and Ribozyme | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Mustafina, Kamila
× Mustafina, Kamila× Fukunaga, Keisuke× Yokobayashi, Yohei |
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書誌情報 |
en : ACS Synthetic Biology 巻 9, 号 1, p. 19-25, 発行日 2019-12-10 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Self-cleaving ribozymes engineered to be activated or inhibited by a small molecule binding to an RNA aptamer inserted within a ribozyme (aptazymes) have proven to be useful for controlling gene expression in living cells. In mammalian cells, an aptazyme embedded in the 5′ or 3′ untranslated region of an mRNA functions as a synthetic riboswitch to chemically regulate gene expression. However, the variety of aptazyme architectures and the ribozyme scaffolds that have been used for mammalian riboswitches has been limited. In particular, fewer synthetic riboswitches that activate gene expression in response to a small molecule (ON-switches) in mammalian cells have been reported compared to OFF-switches. In this work, we developed mammalian riboswitches that function as guanine-activated ON-switches based on a novel aptazyme architecture in which an aptamer and a ribozyme are fused in tandem. The riboswitch performance was optimized by fine-tuning the stability of a critical stem that controls the ribozyme structure and function, yielding switches with ON/OFF ratios greater than 6.0. Our new aptazyme architecture expands the RNA device toolbox for controlling gene expression in mammalian cells. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2161-5063 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/31820936 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acssynbio.9b00371 | |||||
権利 | ||||||
権利情報 | © 2019 American Chemical Society | |||||
権利 | ||||||
権利情報 | ACS AuthorChoice with CC-BY-NC-ND | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/abs/10.1021/acssynbio.9b00371 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |