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Finding cell-specific expression patterns in the early Ciona embryo with single-cell RNA-seq
https://oist.repo.nii.ac.jp/records/1495
https://oist.repo.nii.ac.jp/records/1495cd263418-9322-4be5-a9d8-2f2f7b22f5bf
名前 / ファイル | ライセンス | アクション |
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Ilsley-2020-Finding cell-specific expression p (2.0 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-05-14 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Finding cell-specific expression patterns in the early Ciona embryo with single-cell RNA-seq | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Ilsley, Garth R.
× Ilsley, Garth R.× Suyama, Ritsuko× Noda, Takeshi× Satoh, Nori× Luscombe, Nicholas M. |
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書誌情報 |
en : Scientific Reports 巻 10, 号 1, p. 4961, 発行日 2020-03-18 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Single-cell RNA-seq has been established as a reliable and accessible technique enabling new types of analyses, such as identifying cell types and studying spatial and temporal gene expression variation and change at single-cell resolution. Recently, single-cell RNA-seq has been applied to developing embryos, which offers great potential for finding and characterising genes controlling the course of development along with their expression patterns. In this study, we applied single-cell RNA-seq to the 16-cell stage of the Ciona embryo, a marine chordate and performed a computational search for cell-specific gene expression patterns. We recovered many known expression patterns from our single-cell RNA-seq data and despite extensive previous screens, we succeeded in finding new cell-specific patterns, which we validated by in situ and single-cell qPCR. | |||||
出版者 | ||||||
出版者 | Nature Research | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2045-2322 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32188910 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1038/s41598-020-61591-1 | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s) | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/licenses/by/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.nature.com/articles/s41598-020-61591-1 |