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Potent and broad anticancer activities of leaf extracts from Melia azedarach L. of the subtropical Okinawa islands
https://oist.repo.nii.ac.jp/records/1517
https://oist.repo.nii.ac.jp/records/151785f925a6-9411-45d3-bafc-4fe176b339a9
名前 / ファイル | ライセンス | アクション |
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ajcr0107151 (1.4 MB)
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CC BY-NC 4.0 Creative Commons Attribution-NonCommercial 4.0 International(https://creativecommons.org/licenses/by-nc/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-05-19 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Potent and broad anticancer activities of leaf extracts from Melia azedarach L. of the subtropical Okinawa islands | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Nerome, Kuniaki
× Nerome, Kuniaki× Ito-Kureha, Taku× Paganini, Tiziana× Fukuda, Takao× Igarashi, Yasuhiro× Ashitomi, Hiroto× Ikematsu, Shinya× Yamamoto, Tadashi |
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書誌情報 |
en : American Journal of Cancer Research 巻 10, 号 2, p. 581-594, 発行日 2020-02-01 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Plant extracts have been traditionally used for various therapeutic applications. By conducting an initial screening of several subtropical plants, in this study, we evaluated the anticancer activities of Melia azedarach L. The extract from Melia azedarach L. leaves (MLE) show high cytotoxic effects on cancer cells and in vivo mouse and dog tumor models. During the initial screening, MLE showed strong antiproliferative activity against HT-29 colon, A549 lung, and MKN1 gastric cancer cells. In subsequent tests, using 39 human tumor cell lines, we confirmed the potent anticancer activities of MLE. The anticancer activity of MLE was also confirmed in vivo. MLE markedly inhibited the growth of transplanted gastric MKN1 cancer xenografts in mice. To elucidate the mechanism underlying the anticancer effects of MLE, MLE-treated MKN1 cells were observed using an electron microscope; MLE treatment induced autophagy. Furthermore, western blot analysis of proteins in lysates of MLE-treated cells revealed induction of light chain 3 (LC3)-II autophagosomal proteins. Thus, MLE appeared to suppress MKN1 cell proliferation by inducing autophagy. In addition, in the mouse macrophage cell line J774A.1, MLE treatment induced TNF-alpha production, which might play a role in tumor growth suppression in vivo. We also performed a preclinical evaluation of MLE treatment on dogs with various cancers in veterinary hospitals. Dogs with various types of cancers showed a mean recovery of 76% when treated with MLE. Finally, we tried to identify the active substances present in MLE. All the active fractions obtained by reverse-phase chromatography contained azedarachin B-related moieties, such as 3-deacetyl-12-hydroxy-amoorastatin, 12-hydroxy-amoorastatin, and 12-hydroxyamoorastaton. In conclusion, MLE contains substances with promising anticancer effects, suggesting their future use as safe and effective anticancer agents. | |||||
出版者 | ||||||
出版者 | e-Century Publishing | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2156-6976 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32195029 | |||||
情報源 | ||||||
関連名称 | http://ajcr.us/Instructions.html | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/licenses/by-nc/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://ajcr.us/AJCR_V10N2.html | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7061759/ | |||||
関連名称 | PMC | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |