WEKO3
アイテム
{"_buckets": {"deposit": "096a57cd-40ed-46fc-a223-36cf339cab92"}, "_deposit": {"created_by": 29, "id": "1666", "owners": [29], "pid": {"revision_id": 0, "type": "depid", "value": "1666"}, "status": "published"}, "_oai": {"id": "oai:oist.repo.nii.ac.jp:00001666", "sets": ["40"]}, "author_link": ["10006", "10005", "10003", "10002", "10004"], "item_10001_biblio_info_7": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-06-16", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "160", "bibliographicPageStart": "e61279", "bibliographic_titles": [{}, {"bibliographic_title": "Journal of Visualized Experiments", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "Author", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Masunaga, Aki"}], "nameIdentifiers": [{"nameIdentifier": "10002", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Liu, Andrew W."}], "nameIdentifiers": [{"nameIdentifier": "10003", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tan, Yongkai"}], "nameIdentifiers": [{"nameIdentifier": "10004", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Scott, Andrew"}], "nameIdentifiers": [{"nameIdentifier": "10005", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Luscombe, Nicholas M."}], "nameIdentifiers": [{"nameIdentifier": "10006", "nameIdentifierScheme": "WEKO"}]}]}, "item_10001_description_5": {"attribute_name": "Abstract", "attribute_value_mlt": [{"subitem_description": "Oikopleura dioica is a planktonic chordate with exceptional filter-feeding ability, rapid generation time, conserved early development, and a compact genome. For these reasons, it is considered a useful model organism for marine ecological studies, evolutionary developmental biology, and genomics. As research often requires a steady supply of animal resources, it is useful to establish a reliable, low-maintenance culture system. Here we describe a step-by-step method for establishing an O. dioica culture. We describe how to select potential sampling sites, collection methods, target animal identification, and the set-up of the culturing system. We provide troubleshooting advice based on our own experiences. We also highlight critical factors that help sustain a robust culture system. Although the culture protocol provided here is optimized for O. dioica, we hope our sampling technique and culture setup will inspire new ideas for maintaining other fragile pelagic invertebrates.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_publisher": "MyJove Corporation"}]}, "item_10001_relation_13": {"attribute_name": "PubMedNo.", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:pmid/32628172", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.3791/61279", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_17": {"attribute_name": "Related site", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.jove.com/t/61279/streamlined-sampling-cultivation-pelagic-cosmopolitan-larvacean", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_rights": "© 2020 JoVE"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1940-087X", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "Author\u0027s flag", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Masunaga-2020-Streamlined Sampling and Cultiva.pdf", "filesize": [{"value": "1.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1300000.0, "url": {"label": "Masunaga-2020-Streamlined Sampling and Cultiva", "url": "https://oist.repo.nii.ac.jp/record/1666/files/Masunaga-2020-Streamlined Sampling and Cultiva.pdf"}, "version_id": "c35673e6-5227-4720-b069-0ae088e6cebf"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "29", "path": ["40"], "permalink_uri": "https://oist.repo.nii.ac.jp/records/1666", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-08-06"}, "publish_date": "2020-08-06", "publish_status": "0", "recid": "1666", "relation": {}, "relation_version_is_last": true, "title": ["Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica"], "weko_shared_id": 29}
Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica
https://oist.repo.nii.ac.jp/records/1666
https://oist.repo.nii.ac.jp/records/16663998046c-6388-450b-9b63-4eadae1fed82
名前 / ファイル | ライセンス | アクション |
---|---|---|
Masunaga-2020-Streamlined Sampling and Cultiva (1.3 MB)
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-08-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Streamlined Sampling and Cultivation of the Pelagic Cosmopolitan Larvacean, Oikopleura dioica | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Masunaga, Aki
× Masunaga, Aki× Liu, Andrew W.× Tan, Yongkai× Scott, Andrew× Luscombe, Nicholas M. |
|||||
書誌情報 |
en : Journal of Visualized Experiments 号 160, p. e61279, 発行日 2020-06-16 |
|||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Oikopleura dioica is a planktonic chordate with exceptional filter-feeding ability, rapid generation time, conserved early development, and a compact genome. For these reasons, it is considered a useful model organism for marine ecological studies, evolutionary developmental biology, and genomics. As research often requires a steady supply of animal resources, it is useful to establish a reliable, low-maintenance culture system. Here we describe a step-by-step method for establishing an O. dioica culture. We describe how to select potential sampling sites, collection methods, target animal identification, and the set-up of the culturing system. We provide troubleshooting advice based on our own experiences. We also highlight critical factors that help sustain a robust culture system. Although the culture protocol provided here is optimized for O. dioica, we hope our sampling technique and culture setup will inspire new ideas for maintaining other fragile pelagic invertebrates. | |||||
出版者 | ||||||
出版者 | MyJove Corporation | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1940-087X | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32628172 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3791/61279 | |||||
権利 | ||||||
権利情報 | © 2020 JoVE | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.jove.com/t/61279/streamlined-sampling-cultivation-pelagic-cosmopolitan-larvacean | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |