WEKO3
アイテム
{"_buckets": {"deposit": "56149573-315d-4f65-9280-590d68ee7eaa"}, "_deposit": {"created_by": 27, "id": "1854", "owners": [27], "pid": {"revision_id": 0, "type": "depid", "value": "1854"}, "status": "published"}, "_oai": {"id": "oai:oist.repo.nii.ac.jp:00001854", "sets": ["67"]}, "author_link": ["6955", "10137", "359", "11070", "11073", "11071", "11072"], "item_10001_biblio_info_7": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-08-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "2386", "bibliographicPageStart": "2371", "bibliographicVolumeNumber": "10", "bibliographic_titles": [{}, {"bibliographic_title": "American Journal of Cancer Research", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "Author", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Sai, Sai"}], "nameIdentifiers": [{"nameIdentifier": "11070", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kim, Eun Ho"}], "nameIdentifiers": [{"nameIdentifier": "6955", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Vares, Guillaume"}], "nameIdentifiers": [{"nameIdentifier": "359", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Masao"}], "nameIdentifiers": [{"nameIdentifier": "10137", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yu, Dong"}], "nameIdentifiers": [{"nameIdentifier": "11071", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Horimoto, Yoshiya"}], "nameIdentifiers": [{"nameIdentifier": "11072", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayashi, Mitsuhiro"}], "nameIdentifiers": [{"nameIdentifier": "11073", "nameIdentifierScheme": "WEKO"}]}]}, "item_10001_description_5": {"attribute_name": "Abstract", "attribute_value_mlt": [{"subitem_description": "To investigate whether carbon-ion beam alone, or in combination with lapatinib, has a beneficial effect in targeting HER2-positive breast cancer stem-like cells (CSCs) compared to that of X-rays, human breast CSCs derived from BT474 and SKBR3 cell lines were treated with a carbon-ion beam or X-rays irradiation alone or in combination with lapatinib, and then cell viability, spheroid formation assays, apoptotic analyses, gene expression analysis of related genes, and immunofluorescent gamma-H2AX foci assays were performed. Spheroid formation assays confirmed that ESA+/CD24- cells have CSC properties compared to ESA-/CD24+ cells. CSCs were more highly enriched after X-ray irradiation combined with lapatinib, whereas carbon-ion beam combined with lapatinib significantly decreased the proportion of CSCs. Carbon-ion beam combined with lapatinib significantly suppressed spheroid formation compared to X-rays combined with lapatinib or carbon ion beam alone. Cell cycle analysis showed that carbon ion beam combined with lapatinib predominantly enhanced sub-G1 and G2/M arrested population compared to that of carbon-ion beam, X-ray treatments alone. Carbon-ion beam combined with lapatinib significantly enhanced apoptosis and carbon-ion beam alone dose-dependently increased autophagy-related expression of Beclin1 and in combination with lapatinib greatly enhanced ATG7 expression at protein levels. In addition, a large sized gamma H2AX foci in CSCs were induced by carbon ion beam combined with lapatinib treatment in CSCs compared to cells receiving X-rays or carbon-ion beam alone. Altogether, combination of carbon-ion beam irradiation and lapatinib has a high potential to kill HER2-positive breast CSCs, causing severe irreparable DNA damage, enhanced autophagy, and apoptosis.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_publisher": " e-Century Publishing"}]}, "item_10001_relation_13": {"attribute_name": "PubMedNo.", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:pmid/32905515", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_17": {"attribute_name": "Related site", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.ajcr.us/AJCR_V10N8.html", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_rights": "© 2020 e-Century Publishing"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2156-6976", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "Author\u0027s flag", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-24"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "ajcr0010-2371.pdf", "filesize": [{"value": "3.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/deed.en)", "licensetype": "license_free", "mimetype": "application/pdf", "size": 3500000.0, "url": {"label": "ajcr0010-2371", "url": "https://oist.repo.nii.ac.jp/record/1854/files/ajcr0010-2371.pdf"}, "version_id": "b4606214-496b-43dd-b812-f792d99b1012"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Combination of carbon-ion beam and dual tyrosine kinase inhibitor, lapatinib, effectively destroys HER2 positive breast cancer stem-like cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Combination of carbon-ion beam and dual tyrosine kinase inhibitor, lapatinib, effectively destroys HER2 positive breast cancer stem-like cells", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "27", "path": ["67"], "permalink_uri": "https://oist.repo.nii.ac.jp/records/1854", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-12-24"}, "publish_date": "2020-12-24", "publish_status": "0", "recid": "1854", "relation": {}, "relation_version_is_last": true, "title": ["Combination of carbon-ion beam and dual tyrosine kinase inhibitor, lapatinib, effectively destroys HER2 positive breast cancer stem-like cells"], "weko_shared_id": 27}
Combination of carbon-ion beam and dual tyrosine kinase inhibitor, lapatinib, effectively destroys HER2 positive breast cancer stem-like cells
https://oist.repo.nii.ac.jp/records/1854
https://oist.repo.nii.ac.jp/records/1854ff4bcec6-c505-454f-8925-03850f36cf36
名前 / ファイル | ライセンス | アクション |
---|---|---|
ajcr0010-2371 (3.5 MB)
|
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/deed.en)
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-12-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Combination of carbon-ion beam and dual tyrosine kinase inhibitor, lapatinib, effectively destroys HER2 positive breast cancer stem-like cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Sai, Sai
× Sai, Sai× Kim, Eun Ho× Vares, Guillaume× Suzuki, Masao× Yu, Dong× Horimoto, Yoshiya× Hayashi, Mitsuhiro |
|||||
書誌情報 |
en : American Journal of Cancer Research 巻 10, 号 8, p. 2371-2386, 発行日 2020-08-01 |
|||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | To investigate whether carbon-ion beam alone, or in combination with lapatinib, has a beneficial effect in targeting HER2-positive breast cancer stem-like cells (CSCs) compared to that of X-rays, human breast CSCs derived from BT474 and SKBR3 cell lines were treated with a carbon-ion beam or X-rays irradiation alone or in combination with lapatinib, and then cell viability, spheroid formation assays, apoptotic analyses, gene expression analysis of related genes, and immunofluorescent gamma-H2AX foci assays were performed. Spheroid formation assays confirmed that ESA+/CD24- cells have CSC properties compared to ESA-/CD24+ cells. CSCs were more highly enriched after X-ray irradiation combined with lapatinib, whereas carbon-ion beam combined with lapatinib significantly decreased the proportion of CSCs. Carbon-ion beam combined with lapatinib significantly suppressed spheroid formation compared to X-rays combined with lapatinib or carbon ion beam alone. Cell cycle analysis showed that carbon ion beam combined with lapatinib predominantly enhanced sub-G1 and G2/M arrested population compared to that of carbon-ion beam, X-ray treatments alone. Carbon-ion beam combined with lapatinib significantly enhanced apoptosis and carbon-ion beam alone dose-dependently increased autophagy-related expression of Beclin1 and in combination with lapatinib greatly enhanced ATG7 expression at protein levels. In addition, a large sized gamma H2AX foci in CSCs were induced by carbon ion beam combined with lapatinib treatment in CSCs compared to cells receiving X-rays or carbon-ion beam alone. Altogether, combination of carbon-ion beam irradiation and lapatinib has a high potential to kill HER2-positive breast CSCs, causing severe irreparable DNA damage, enhanced autophagy, and apoptosis. | |||||
出版者 | ||||||
出版者 | e-Century Publishing | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2156-6976 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32905515 | |||||
権利 | ||||||
権利情報 | © 2020 e-Century Publishing | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.ajcr.us/AJCR_V10N8.html | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |