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How Do You Identify m⁶A Methylation in Transcriptomes at High Resolution? A Comparison of Recent Datasets
https://oist.repo.nii.ac.jp/records/1860
https://oist.repo.nii.ac.jp/records/1860a73b0646-fea1-473d-90bb-7ef354b1ba1c
名前 / ファイル | ライセンス | アクション |
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Capitanchik-2020-How Do You Identify m(6) A Me (1.9 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-12-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | How Do You Identify m⁶A Methylation in Transcriptomes at High Resolution? A Comparison of Recent Datasets | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | RNA | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | N6-methyladenosine | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | m⁶A | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | epitranscriptomics | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | bioinformatics | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Capitanchik, Charlotte
× Capitanchik, Charlotte× Toolan-Kerr, Patrick× Luscombe, Nicholas M.× Ule, Jernej |
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書誌情報 |
en : Frontiers in Genetics 巻 11, p. 398, 発行日 2020-05-20 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | A flurry of methods has been developed in recent years to identify N6-methyladenosine (m⁶A) sites across transcriptomes at high resolution. This raises the need to understand both the common features and those that are unique to each method. Here, we complement the analyses presented in the original papers by reviewing their various technical aspects and comparing the overlap between m⁶A-methylated messenger RNAs (mRNAs) identified by each. Specifically, we examine eight different methods that identify m⁶A sites in human cells with high resolution: two antibody-based crosslinking and immunoprecipitation (CLIP) approaches, two using endoribonuclease MazF, one based on deamination, two using Nanopore direct RNA sequencing, and finally, one based on computational predictions. We contrast the respective datasets and discuss the challenges in interpreting the overlap between them, including a prominent expression bias in detected genes. This overview will help guide researchers in making informed choices about using the available data and assist with the design of future experiments to expand our understanding of m⁶A and its regulation. | |||||
出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1664-8021 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32508872 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3389/fgene.2020.00398 | |||||
権利 | ||||||
権利情報 | © 2020 Capitanchik, Toolan-Kerr, Luscombe and Ule. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.frontiersin.org/articles/10.3389/fgene.2020.00398/full | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |