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High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases
https://oist.repo.nii.ac.jp/records/1897
https://oist.repo.nii.ac.jp/records/18974fb02721-086b-4ab9-ba4a-8e2eea077169
名前 / ファイル | ライセンス | アクション |
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Koyama-Honda-2020-High-speed single-molecule i (4.1 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-01-19 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Koyama-Honda, Ikuko
× Koyama-Honda, Ikuko× Fujiwara, Takahiro K.× Kasai, Rinshi S.× Suzuki, Kenichi G.N.× Kajikawa, Eriko× Tsuboi, Hisae× Tsunoyama, Taka A.× Kusumi, Akihiro |
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書誌情報 |
en : Journal of Cell Biology 巻 219, 号 12, p. e202006125, 発行日 2020-12-07 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Using single-molecule imaging with enhanced time resolutions down to 5 ms, we found that CD59 cluster rafts and GM1 cluster rafts were stably induced in the outer leaflet of the plasma membrane (PM), which triggered the activation of Lyn, H-Ras, and ERK and continually recruited Lyn and H-Ras right beneath them in the inner leaflet with dwell lifetimes <0.1 s. The detection was possible due to the enhanced time resolutions employed here. The recruitment depended on the PM cholesterol and saturated alkyl chains of Lyn and H-Ras, whereas it was blocked by the nonraftophilic transmembrane protein moiety and unsaturated alkyl chains linked to the inner-leaflet molecules. Because GM1 cluster rafts recruited Lyn and H-Ras as efficiently as CD59 cluster rafts, and because the protein moieties of Lyn and H-Ras were not required for the recruitment, we conclude that the transbilayer raft phases induced by the outer-leaflet stabilized rafts recruit lipid-anchored signaling molecules by lateral raft-lipid interactions and thus serve as a key signal transduction platform. | |||||
出版者 | ||||||
出版者 | Rockefeller University Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0021-9525 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1540-8140 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/33053147 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1083/jcb.202006125 | |||||
権利 | ||||||
権利情報 | © 2020 Koyama-Honda et al. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://rupress.org/jcb/article-standard/219/12/e202006125/211461/High-speed-single-molecule-imaging-reveals-signal | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |