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Direct binding of TFEα opens DNA binding cleft of RNA polymerase
https://oist.repo.nii.ac.jp/records/1947
https://oist.repo.nii.ac.jp/records/19475e0ca311-b70c-4fb1-83b9-4026bb48d6e9
名前 / ファイル | ライセンス | アクション |
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Jun-2020-Direct binding of TFEα opens DNA bind (3.3 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-02-25 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Direct binding of TFEα opens DNA binding cleft of RNA polymerase | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Jun, Sung-Hoon
× Jun, Sung-Hoon× Hyun, Jaekyung× Cha, Jeong Seok× Kim, Hoyoung× Bartlett, Michael S.× Cho, Hyun-Soo× Murakami, Katsuhiko S. |
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書誌情報 |
en : Nature Communications 巻 11, 号 1, p. 6123, 発行日 2020-11-30 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Opening of the DNA binding cleft of cellular RNA polymerase (RNAP) is necessary for transcription initiation but the underlying molecular mechanism is not known. Here, we report on the cryo-electron microscopy structures of the RNAP, RNAP-TFEα binary, and RNAP-TFEα-promoter DNA ternary complexes from archaea, Thermococcus kodakarensis (Tko). The structures reveal that TFEα bridges the RNAP clamp and stalk domains to open the DNA binding cleft. Positioning of promoter DNA into the cleft closes it while maintaining the TFEα interactions with the RNAP mobile modules. The structures and photo-crosslinking results also suggest that the conserved aromatic residue in the extended winged-helix domain of TFEα interacts with promoter DNA to stabilize the transcription bubble. This study provides a structural basis for the functions of TFEα and elucidates the mechanism by which the DNA binding cleft is opened during transcription initiation in the stalk-containing RNAPs, including archaeal and eukaryotic RNAPs. | |||||
出版者 | ||||||
出版者 | Nature Research | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2041-1723 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/33257704 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1038/s41467-020-19998-x | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.nature.com/articles/s41467-020-19998-x | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |