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後生動物のベータカテニン多機能化の進化に関する研究
https://doi.org/10.15102/1394.00002299
https://doi.org/10.15102/1394.000022990c3f4a63-3241-4bac-8f2c-33c36665312c
名前 / ファイル | ライセンス | アクション |
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FullText-Mbongo (11.7 MB)
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MbogoFinalExamAbstract (183.3 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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公開日 | 2022-02-14 | |||||||
タイトル | ||||||||
言語 | ja | |||||||
タイトル | 後生動物のベータカテニン多機能化の進化に関する研究 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | The Evolution of Dual Functionality of β-catenin in Metazoans | |||||||
言語 | ||||||||
言語 | eng | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
資源タイプ | doctoral thesis | |||||||
ID登録 | ||||||||
ID登録 | 10.15102/1394.00002299 | |||||||
ID登録タイプ | JaLC | |||||||
アクセス権 | ||||||||
アクセス権 | open access | |||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
著者 (英) |
Mbogo, Ivan
× Mbogo, Ivan
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抄録 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | The evolution of the multicellular body animals from unicellular organisms is still a significant and long-lasting subject of interest in biology. Acquisition of cell-cell adhesion with cadherin, α- and β-catenin proteins is thought to be tightly coupled with the origin of animal epithelium and consequent evolutionary thrive of animals. On the other hand, much research has shown, in a wide range of animal lineages such as bilaterians and cnidarians, that β-catenin associates with diverse intracellular proteins involved in gene transcription/translation and plays an essential role in the induction of the signalling centre (organiser) during animal embryogenesis. The pleiotropic and evolutionary conserved functions of β-catenin suggest deep evolutionary roots of the β-catenin complexes and involvement in the emergence of basic animal body plan. Recent progress in genomics has identified genes of the cell-cell adhesion complex and signalling machinery of β-catenin in genomes of early-branching animals, including Porifera (sponges) and Ctenophora(comb jellies). However, due to difficulties in applying molecular and genetic technologies in these non-model animals, the ancestral functions of β-catenin complexes remain largely to be explored.In this study, I combined structural, proteomic, and functional approaches to understand evolutionarily conserved features of the β-catenin and its associated proteins. Structural analysis suggests a unicellular origin of the basic architecture of β-catenin protein, while amino acid residues critical in adhesive properties are conserved only within animals. To analyse evolutionarily conserved functional characteristics of basal animal β-catenins, I performed transphyletic studies where the basal animal β-catenins are expressed in Xenopus embryos. A series of proteomics analyses of β-catenin-associated proteins revealed the cadherin catenin complex's deep origin and evolutionary conservation. The transphyletic function studies and detailed sequence analysis also revealed the β-catenin's organiser-inducing function of Cnidaria, Porifera, but not Ctenophora. These data suggest that the primary function of ancestral β-catenin was to play adhesive roles, and its' signalling properties were equipped later during the evolution of basal animals. | |||||||
口頭試問日 | ||||||||
2021-11-19 | ||||||||
学位授与年月日 | ||||||||
学位授与年月日 | 2021-12-31 | |||||||
学位名 | ||||||||
学位名 | Doctor of Philosophy | |||||||
学位授与番号 | ||||||||
学位授与番号 | 甲第83号 | |||||||
学位授与機関 | ||||||||
学位授与機関識別子Scheme | kakenhi | |||||||
学位授与機関識別子 | 38005 | |||||||
学位授与機関名 | Okinawa Institute of Science and Technology Graduate University | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
権利 | ||||||||
権利情報 | © 2021 The Author. |