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Regulation of CCR4-NOT complex deadenylase activity and cellular responses by MK2-dependent phosphorylation of CNOT2
https://oist.repo.nii.ac.jp/records/2599
https://oist.repo.nii.ac.jp/records/2599f1832441-bc0e-436a-a1d8-9b6d766b17f3
名前 / ファイル | ライセンス | アクション |
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Regulation of CCR4 NOT complex deadenylase activity and cellular responses by MK2 dependent phosphorylation of CNOT2 (7.1 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-03-22 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Regulation of CCR4-NOT complex deadenylase activity and cellular responses by MK2-dependent phosphorylation of CNOT2 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | CCR4-NOT complex | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | MK2 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | mRNA decay | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | phosphorylation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | stress response | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Suzuki, Toru
× Suzuki, Toru× Hoshina, Miyuki× Nishijima, Saori× Hoshina, Naosuke× Kikuguchi, Chisato× Tomohiro, Takumi× Fukao, Akira× Fujiwara, Toshinobu× Yamamoto, Tadashi |
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書誌情報 |
en : RNA Biology 巻 19, 号 1, p. 234-246, 発行日 2022-02-06 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | CCR4-NOT complex-mediated mRNA deadenylation serves critical functions in multiple biological processes, yet how this activity is regulated is not fully understood. Here, we show that osmotic stress induces MAPKAPK-2 (MK2)-mediated phosphorylation of CNOT2. Programmed cell death is greatly enhanced by osmotic stress in CNOT2-depleted cells, indicating that CNOT2 is responsible for stress resistance of cells. Although wild-type (WT) and non-phosphorylatable CNOT2 mutants reverse this sensitivity, a phosphomimetic form of CNOT2, in which serine at the phosphorylation site is replaced with glutamate, does not have this function. We also show that mRNAs have elongated poly(A) tails in CNOT2-depleted cells and that introduction of CNOT2 WT or a non-phosphorylatable mutant, but not phosphomimetic CNOT2, renders their poly(A) tail lengths comparable to those in control HeLa cells. Consistent with this, the CCR4-NOT complex containing phosphomimetic CNOT2 exhibits less deadenylase activity than that containing CNOT2 WT. These data suggest that CCR4-NOT complex deadenylase activity is regulated by post-translational modification, yielding dynamic control of mRNA deadenylation. | |||||
出版者 | ||||||
出版者 | Informa UK Limited, trading as Taylor & Francis Group. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1547-6286 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1555-8584 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35129087 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1080/15476286.2021.2021676 | |||||
権利 | ||||||
権利情報 | © 2022 The Authors. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.tandfonline.com/doi/full/10.1080/15476286.2021.2021676 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |