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Sequestration and efflux largely account for cadmium and copper resistance in the deep‐sea Nitratiruptor sp. SB155‐2 (phylum Campylobacterota)
https://oist.repo.nii.ac.jp/records/2829
https://oist.repo.nii.ac.jp/records/28290d1d3bd7-c293-4227-bb23-4e15c23ca642
名前 / ファイル | ライセンス | アクション |
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Ares-Sequestration and efflux largely account_vor (1.6 MB)
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CC BY 4.0
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-10-31 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Sequestration and efflux largely account for cadmium and copper resistance in the deep‐sea Nitratiruptor sp. SB155‐2 (phylum Campylobacterota) | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Ares, Ángela
× Ares, Ángela× Sakai, Sanae× Sasaki, Toshio× Shimamura, Shigeru× Mitarai, Satoshi× Nunoura, Takuro |
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書誌情報 |
en : Environmental Microbiology 発行日 2022-10-28 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | In deep-sea hydrothermal vent environments, metal-enriched fluids and sediments abound, making these habitats ideal to study metal resistance in prokaryotes. In this investigation, we employed transcriptomics and shotgun proteomics with scanning transmission electron microscopy and energy-dispersive x-ray spectroscopy (STEM-EDX) to better understand mechanisms of tolerance for cadmium (Cd) and copper (Cu) at stress-inducing concentrations in Nitratiruptor sp. SB155-2 (phylum Campylobacterota). Transcriptomic profiles were remarkably different in the presence of these two metals, displaying 385 (19%) and 629 (31%) differentially transcribed genes (DTG) in the presence of Cd(II) and Cu(II), respectively, while only 7% of differentially transcribed (DT) genes were shared, with genes for non-specific metal transporters and genes involved in oxidative stress-response predominating. Transcriptomic and proteomic analyses confirmed that metal-specific DT pathways under Cu(II) stress, including those involving sulfur, cysteine, and methionine, are likely required for high-affinity efflux systems, while flagella formation and chemotaxis were over-represented under Cd(II) stress. Consistent with these differences, STEM-EDX analysis revealed that polyphosphate-like granules (pPLG), the formation of CdS particles, and the periplasmic space are crucial for Cd(II) sequestration. Overall, this study provides new insights regarding metal-specific adaptations of Campylobacterota to deep-sea hydrothermal vent environments. | |||||
出版者 | ||||||
出版者 | Applied Microbiology International | |||||
出版者 | ||||||
出版者 | John Wiley & Sons Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1462-2912 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1462-2920 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/36284406 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1111/1462-2920.16255 | |||||
権利 | ||||||
権利情報 | © 2022 The Authors. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.16255 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |