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Controlling Bdellovibrio bacteriovorus Gene Expression and Predation Using Synthetic Riboswitches
https://oist.repo.nii.ac.jp/records/356
https://oist.repo.nii.ac.jp/records/356fb6b71f7-0e64-4b05-8c9a-5d872c242400
名前 / ファイル | ライセンス | アクション |
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acssynbio.7b00171 (2.6 MB)
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ACS AuthorChoice (https://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-04-10 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Controlling Bdellovibrio bacteriovorus Gene Expression and Predation Using Synthetic Riboswitches | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Dwidar, Mohammed
× Dwidar, Mohammed× Yokobayashi, Yohei |
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書誌情報 |
en : ACS Synthetic Biology 巻 6, 号 11, p. 2035-2041, 発行日 2017-08-16 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Bdellovibrio bacteriovorus is a predatory bacterium that feeds on Gram-negative bacteria including a wide range of pathogens and thus has potential applications as a biocontrol agent. Owing to its unique life cycle, however, there are limited tools that enable genetic manipulation of B. bacteriovorus. This work describes our first steps toward engineering the predatory bacterium for practical applications by developing basic genetic parts to control gene expression. Specifically, we evaluated four robust promoters that are active during the attack phase of B. bacteriovorus. Subsequently, we tested several synthetic riboswitches that have been reported to function in Escherichia coli, and identified theophylline-activated riboswitches that function in B. bacteriovorus. Finally, we inserted the riboswitch into the bacterial chromosome to regulate expression of the flagellar sigma factor fliA, which was previously predicted to be essential for predation, and observed that the engineered strain shows a faster predation kinetics in the presence of theophylline. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2161-5063 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/28812884 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acssynbio.7b00171 | |||||
権利 | ||||||
権利情報 | © 2017 American Chemical Society | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1021/acssynbio.7b00171 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |