WEKO3
アイテム
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Insights into gene expression profiles induced by Socs3 depletion in keratinocytes
https://oist.repo.nii.ac.jp/records/360
https://oist.repo.nii.ac.jp/records/3608a1156be-5fba-4533-aa94-7ecee4de26ba
名前 / ファイル | ライセンス | アクション |
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s41598-017-16155-1 (2.7 MB)
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Creative Commons Attribution 4.0 International
(http://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-04-11 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Insights into gene expression profiles induced by Socs3 depletion in keratinocytes | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Bajpai, Archana
× Bajpai, Archana× Ishii, Takashi× Miyauchi, Kosuke× Gupta, Vipul× Nishio-Masaike, Yuka× Shimizu-Yoshida, Yuki× Kubo, Masato× Kitano, Hiroaki |
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書誌情報 |
en : Scientific Reports 巻 7, 号 1, p. 15830, 発行日 2017-11-20 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Specific deletion of suppressor of cytokine signaling 3 (Socs3) in keratinocytes can cause severe skin inflammation with infiltration of immune cells. The molecular mechanisms and key regulatory pathways involved in these processes remain elusive. To investigate the role of Socs3 in keratinocytes, we generated and analyzed global RNA-Seq profiles from Socs3 conditional knockout (cKO) mice of two different ages (2 and 10 weeks). Over 400 genes were significantly regulated at both time points. Samples from 2-week-old mice exhibited down-regulation of genes involved in keratin-related functions and up-regulation of genes involved in lipid metabolism. At week 10, multiple chemokine and cytokine genes were up-regulated. Functional annotation revealed that the genes differentially expressed in the 2-week-old mice play roles in keratinization, keratinocyte differentiation, and epidermal cell differentiation. By contrast, differentially expressed genes in the 10-week-old animals are involved in acute immune-related functions. A group of activator protein-1–related genes were highly up-regulated in Socs3 cKO mice of both ages. This observation was validated using qRT-PCR by SOCS3-depleted human keratinocyte–derived HaCaT cells. Our results suggest that, in addition to participating in immune-mediated pathways, SOCS3 also plays important roles in skin barrier homeostasis. | |||||
出版者 | ||||||
出版者 | Springer Nature | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2045-2322 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/29158586 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1038/s41598-017-16155-1 | |||||
権利 | ||||||
権利情報 | © 2017 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1038/s41598-017-16155-1 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |