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Development of new ganglioside probes and unraveling of raft domain structure by single-molecule imaging
https://oist.repo.nii.ac.jp/records/883
https://oist.repo.nii.ac.jp/records/8830058aefb-61c9-413d-873c-e17d3bef6f65
名前 / ファイル | ライセンス | アクション |
---|---|---|
BBA-最終版-2 (2.9 MB)
|
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(http://creativecommons.org/Licenses/by-nc-nd/ 4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-05-21 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Development of new ganglioside probes and unraveling of raft domain structure by single-molecule imaging | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Suzuki, Kenichi G.N.
× Suzuki, Kenichi G.N.× Ando, Hiromune× Komura, Naoko× Fujiwara, Takahiro K.× Kiso, Makoto× Kusumi, Akihiro |
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書誌情報 |
en : Biochimica et Biophysica Acta (BBA) - General Subjects 巻 1861, 号 10, p. 2494-2506, 発行日 2017-07-20 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Gangliosides are involved in a variety of biological roles and are a component of lipid rafts found in cell plasma membranes (PMs). Gangliosides are especially abundant in neuronal PMs and are essential to their physiological functions. However, the dynamic behaviors of gangliosides have not been investigated in living cells due to a lack of fluorescent probes that behave like their parental molecules. We have recently developed, using an entirely chemical method, four new ganglioside probes (GM1, GM2, GM3, and GD1b) that act similarly to their parental molecules in terms of raft partitioning and binding affinity. Using single fluorescent-molecule imaging, we have found that ganglioside probes dynamically enter and leave rafts featuring CD59, a GPI-anchored protein. This occurs both before and after stimulation. The residency time of our ganglioside probes in rafts with CD59 oligomers was 48 ms, after stimulation. The residency times in CD59 homodimer and monomer rafts were 40 ms and 12 ms, respectively. In this review, we introduce an entirely chemical-based ganglioside analog synthesis method and describe its application in single-molecule imaging and for the study of the dynamic behavior of gangliosides in cell PMs. Finally, we discuss how raft domains are formed, both before and after receptor engagement. This article is part of a Special Issue entitled Neuro-glycoscience, edited by Kenji Kadomatsu and Hiroshi Kitagawa. | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0304-4165 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/28734966 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.bbagen.2017.07.012 | |||||
権利 | ||||||
権利情報 | © 2017 Elsevier B.V. | |||||
情報源 | ||||||
関連名称 | http://creativecommons.org/Licenses/by-nc-nd/ 4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S0304416517302234?via%3Dihub | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |