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High‐resolution archaellum structure reveals a conserved metal‐binding site
https://oist.repo.nii.ac.jp/records/1027
https://oist.repo.nii.ac.jp/records/1027d05d9a89-6b5c-4890-b055-ef18d8f13f60
名前 / ファイル | ライセンス | アクション |
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Meshcheryakov-2019-High-resolution archaellum (2.8 MB)
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Creative Commons
Attribution-NonCommercial-NoDerivatives 4.0 International (https://creativecommons.org/licenses/by-nc-nd/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-07-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | High‐resolution archaellum structure reveals a conserved metal‐binding site | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Meshcheryakov, Vladimir A
× Meshcheryakov, Vladimir A× Shibata, Satoshi× Schreiber, Makoto Tokoro× Villar‐Briones, Alejandro× Jarrell, Kenneth F× Aizawa, Shin‐Ichi× Wolf, Matthias |
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書誌情報 |
en : EMBO reports 巻 20, 号 5, p. e46340, 発行日 2019-03-21 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Many archaea swim by means of archaella. While the archaellum is similar in function to its bacterial counterpart, its structure, composition, and evolution are fundamentally different. Archaella are related to archaeal and bacterial type IV pili. Despite recent advances, our understanding of molecular processes governing archaellum assembly and stability is still incomplete. Here, we determine the structures of Methanococcus archaella by X-ray crystallography and cryo-EM. The crystal structure of Methanocaldococcus jannaschii FlaB1 is the first and only crystal structure of any archaellin to date at a resolution of 1.5 angstrom, which is put into biological context by a cryo-EM reconstruction from Methanococcus maripaludis archaella at 4 angstrom resolution created with helical single-particle analysis. Our results indicate that the archaellum is predominantly composed of FlaB1. We identify N-linked glycosylation by cryo-EM and mass spectrometry. The crystal structure reveals a highly conserved metal-binding site, which is validated by mass spectrometry and electron energy-loss spectroscopy. We show invitro that the metal-binding site, which appears to be a widespread property of archaellin, is required for filament integrity. | |||||
出版者 | ||||||
出版者 | EMBO Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1469-221X | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1469-3178 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/30898768 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.15252/embr.201846340 | |||||
権利 | ||||||
権利情報 | © 2019 The Author(s). | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.embopress.org/doi/10.15252/embr.201846340 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |