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マウスバレル皮質顆粒上層における二光子膜電位イメージング:振動と応答
https://doi.org/10.15102/1394.00001041
https://doi.org/10.15102/1394.000010415fc55bed-ab81-493a-b12e-0c68ff02fbd5
名前 / ファイル | ライセンス | アクション |
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Full_Text (9.7 MB)
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Final_Exam_Abstract (42.9 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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公開日 | 2019-11-06 | |||||||
タイトル | ||||||||
言語 | ja | |||||||
タイトル | マウスバレル皮質顆粒上層における二光子膜電位イメージング:振動と応答 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | Two-Photon Voltage Imaging of Supragranular Barrel Cortex in Mice: Oscillations and Responses | |||||||
言語 | ||||||||
言語 | eng | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
資源タイプ | doctoral thesis | |||||||
ID登録 | ||||||||
ID登録 | 10.15102/1394.00001041 | |||||||
ID登録タイプ | JaLC | |||||||
アクセス権 | ||||||||
アクセス権 | open access | |||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
著者 (英) |
Dalphin, Neil
× Dalphin, Neil
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抄録 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | The supragranular layers of cortex are key information integration and computation areas with dominant cortico-cortical connections. While layers 2 and 3 are densely packed with somata, layer 1 is almost free of somata. The absence of somata makes the analysis of layer 1 difficult. Electrical recording from small processes within layer 1 are not possible and electric field recordings are difficult due to the low seal resistance. Imaging processes of layer 1 remains difficult as cells project into it from many distant areas and due to the dense and intermingled packing. Here, I record sensory signals in supragranular layers, including layer 1 through a combination of voltage sensitive dye imaging, and an intracellular calcium indicator. Optical sectioning with two-photon microscopy allowed resolution in depth, showing changes in the sensory signal within layer 1. Additionally, cortical oscillations were detected with the voltage-sensitive dye in the delta, theta, and beta bands, and, for the first time with voltage imaging, also in the slow-gamma (35 Hz) band, in vivo. Delta, theta, and gamma oscillations were modulated by sensory stimuli. As very little is known about membrane voltage oscillations in layer 1 and to optimize optical voltage recordings in layer 1, I developed a novel surgery to apply voltage dye primarily to layer 1, without removing the dura or injecting dye within the brain. I also applied a new voltage-sensitive dye optimized for tissue diffusion with this surgery. I imaged cortical membrane potential oscillations with two-photon microscopy depth-resolved (25 to 100 µm below dura) in anesthetized and awake mice. Again, I found delta (0.5-4 Hz), theta (4-10 Hz), low beta (10-20 Hz), and low gamma (30-40 Hz) oscillations. All oscillation bands were stronger in awake animals. While the power of delta, theta, and low beta oscillations increased with depth, the power of low gamma was more constant throughout layer 1. These findings identify layer 1 as an important coordination hub for the dynamic binding process of neurons mediated by oscillations. |
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言語 | en | |||||||
口頭試問日 | ||||||||
2019-08-21 | ||||||||
学位授与年月日 | ||||||||
学位授与年月日 | 2019-09-30 | |||||||
学位名 | ||||||||
学位名 | Doctor of Philosophy | |||||||
学位授与番号 | ||||||||
学位授与番号 | 甲第37号 | |||||||
学位授与機関 | ||||||||
学位授与機関識別子Scheme | kakenhi | |||||||
学位授与機関識別子 | 38005 | |||||||
学位授与機関名 | Okinawa Institute of Science and Technology Graduate University | |||||||
著者版フラグ | ||||||||
出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
権利 | ||||||||
権利情報 | © 2019 The Author. |