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Nuclear AGO1 Regulates Gene Expression by Affecting Chromatin Architecture in Human Cells
https://oist.repo.nii.ac.jp/records/1166
https://oist.repo.nii.ac.jp/records/11665988cc0f-88f4-4b86-b3f7-4d24f3bb2e69
名前 / ファイル | ライセンス | アクション |
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Shuaib-2019-Nuclear AGO1 Regulates Gene Expres (6.1 MB)
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Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-01-29 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Nuclear AGO1 Regulates Gene Expression by Affecting Chromatin Architecture in Human Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Shuaib, Muhammad
× Shuaib, Muhammad× Parsi, Krishna Mohan× Thimma, Manjula× Adroub, Sabir Abdu× Kawaji, Hideya× Seridi, Loqmane× Ghosheh, Yanal× Fort, Alexandre× Fallatah, Bodor× Ravasi, Timothy× Carninci, Piero× Orlando, Valerio |
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書誌情報 |
en : Cell Systems 巻 9, 号 5, p. 446-458.e6, 発行日 2019-10-16 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The impact of mammalian RNA interference components, particularly, Argonaute proteins, on chromatin organization is unexplored. Recent reports indicate that AGO1 association with chromatin appears to influence gene expression. To uncover the role of AGO1 in the nucleus, we used a combination of genome-wide approaches in control and AGO1-depleted HepG2 cells. We found that AGO1 strongly associates with active enhancers and RNA being produced at those sites. Hi-C analysis revealed AGO1 enrichment at the boundaries of topologically associated domains (TADs). By Hi-C in AGO1 knockdown cells, we observed changes in chromatin organization, including TADs and A/B compartment mixing, specifically in AGO1-bound regions. Distinct groups of genes and especially eRNA transcripts located within differentially interacting loci showed altered expression upon AGO1 depletion. Moreover, AGO1 association with enhancers is dependent on eRNA transcription. Collectively, our data suggest that enhancer-associated AGO1 contributes to the fine-tuning of chromatin architecture and gene expression in human cells. | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2405-4712 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/31629687 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.cels.2019.09.005 | |||||
権利 | ||||||
権利情報 | © 2019 The Author(s). | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/licenses/by/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S2405471219303138?via%3Dihub | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |