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Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch
https://oist.repo.nii.ac.jp/records/1211
https://oist.repo.nii.ac.jp/records/1211100e40c9-9f78-44dd-ab0a-a209d52fae0a
名前 / ファイル | ライセンス | アクション |
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jacs.9b03300 (4.8 MB)
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ACS AuthorChoice
(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-03-02 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Programmable Artificial Cells Using Histamine-Responsive Synthetic Riboswitch | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Dwidar, Mohammed
× Dwidar, Mohammed× Seike, Yusuke× Kobori, Shungo× Whitaker, Charles× Matsuura, Tomoaki× Yokobayashi, Yohei |
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書誌情報 |
en : Journal of the American Chemical Society 巻 141, 号 28, p. 11103-11114, 発行日 2019-06-17 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Artificial cells that encapsulate DNA-programmable protein expression machinery are emerging as an attractive platform for studying fundamental cellular properties and applications in synthetic biology. However, interfacing these artificial cells with the complex and dynamic chemical environment remains a major and urgent challenge. We demonstrate that the repertoire of molecules that artificial cells respond to can be expanded by synthetic RNA-based gene switches, or riboswitches. We isolated an RNA aptamer that binds histamine with high affinity and specificity and used it to design robust riboswitches that activate protein expression in the presence of histamine. Finally, the riboswitches were incorporated in artificial cells to achieve controlled release of an encapsulated small molecule and to implement a self-destructive kill-switch. Synthetic riboswitches should serve as modular and versatile interfaces to link artificial cell phenotypes with the complex chemical environment. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0002-7863 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1520-5126 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/31241330 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/jacs.9b03300 | |||||
権利 | ||||||
権利情報 | © 2019 American Chemical Society. | |||||
情報源 | ||||||
関連名称 | https://pubs.acs.org/page/policy/authorchoice_termsofuse.html | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/abs/10.1021/jacs.9b03300 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |