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Machine-driven parameter screen of biochemical reactions
https://oist.repo.nii.ac.jp/records/1277
https://oist.repo.nii.ac.jp/records/12777f220381-a710-4ba7-aba7-0e934ab3b46c
名前 / ファイル | ライセンス | アクション |
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Poulain-2020-Machine-driven parameter screen o (5.3 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-03-13 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Machine-driven parameter screen of biochemical reactions | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Poulain, Stéphane
× Poulain, Stéphane× Arnaud, Ophélie× Kato, Sachi× Chen, Iris× Ishida, Hiro× Carninci, Piero× Plessy , Charles |
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書誌情報 |
en : Nucleic Acids Research 巻 48, 号 7, p. e37, 発行日 2020-02-06 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The development of complex methods in molecular biology is a laborious, costly, iterative and often intuition-bound process where optima are sought in a multidimensional parameter space through step-by-step optimizations. The difficulty of miniaturizing reactions under the microliter volumes usually handled in multiwell plates by robots, plus the cost of the experiments, limit the number of parameters and the dynamic ranges that can be explored. Nevertheless, because of non-linearities of the response of biochemical systems to their reagent concentrations, broad dynamic ranges are necessary. Here we use a high-performance nanoliter handling platform and computer generation of liquid transfer programs to explore in quadruplicates 648 combinations of 4 parameters of a biochemical reaction, the reverse-transcription, which lead us to uncover non-linear responses, parameter interactions and novel mechanistic insights. With the increased availability of computer-driven laboratory platforms for biotechnology, our results demonstrate the feasibility and advantage of methods development based on reproducible, computer-aided exhaustive characterization of biochemical systems. | |||||
出版者 | ||||||
出版者 | Oxford University Press on behalf of Nucleic Acids Research. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0305-1048 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1362-4962 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32025730 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1093/nar/gkaa079 | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s) | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/licenses/by/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://academic.oup.com/nar/article/48/7/e37/5728495 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |