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Presynaptic Black Box Opened by Pioneers at Biophysics Department in University College London
https://oist.repo.nii.ac.jp/records/1407
https://oist.repo.nii.ac.jp/records/14073c37ac79-0e59-47f2-bfce-22290a1863c8
名前 / ファイル | ライセンス | アクション |
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Takahashi-2019-Presynaptic Black Box Opened by (2.9 MB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (https://creativecommons.org/licenses/by-nc-nd/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-04-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Presynaptic Black Box Opened by Pioneers at Biophysics Department in University College London | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Takahashi, Tomoyuki
× Takahashi, Tomoyuki |
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書誌情報 |
en : Neuroscience 発行日 2019-05-18 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The mechanism of chemical synaptic transmission was elucidated at the frog neuromuscular junction (NMJ) and at the squid giant synapse by Katz, Miledi and other researchers. Later progress in molecular biology revealed numerous types of proteins in mammalian central synapses. To establish molecular-functional correlation in synaptic transmission, it now seems essential to re-address the fundamental mechanisms at mammalian central synapses. Using patch-clamp whole-cell recording at the calyx of Held in slices of rodent brainstem, we have identified quantal EPSCs and reproduced the quantal analysis established at the NMJ. Intra-terminal whole-cell loading of the neurotransmitter, glutamate, revealed that vesicular transmitter content is an important determinant of quantal size and its variation. Regarding the Ca(2+)-dependence of transmitter release, the average coupling distance between Ca(2+) entry sites and exocytic vesicles was estimated as tens of nanometers and was found to undergo developmental tightening at the calyx of Held. Numerical simulations suggested that this distance can determine the synaptic delay, synchronicity of vesicular transmitter release, and release probability. The super-linear input (presynaptic)-output (postsynaptic) relationship of neurotransmission is an important physiological feature discovered at squid giant synapses. However, at the calyx of Held, unlike at the squid synapse, the input-output relationship had a wide safety margin, protecting transmitter release from a diminishment of presynaptic action potentials. As in the NMJ, Ca(2+) remaining in the cytosol after action potential facilitates subsequent release. As a downstream mechanism of this residual Ca(2+), a Ca(2+)-induced Ca(2+) channel activation via high-affinity Ca(2+) binding proteins was discovered at mammalian central synapses. | |||||
出版者 | ||||||
出版者 | Elsevier Ltd on behalf of IBRO | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0306-4522 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/31108128 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.neuroscience.2019.04.029 | |||||
権利 | ||||||
権利情報 | © 2019 The Author(s) | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S0306452219302787 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |