WEKO3
アイテム
{"_buckets": {"deposit": "00967094-9990-4891-b9bd-fd7d0814fcea"}, "_deposit": {"created_by": 28, "id": "1479", "owners": [28], "pid": {"revision_id": 0, "type": "depid", "value": "1479"}, "status": "published"}, "_oai": {"id": "oai:oist.repo.nii.ac.jp:00001479", "sets": ["54"]}, "author_link": ["8947", "8946", "8945"], "item_10001_biblio_info_7": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-01-28", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "5", "bibliographicPageEnd": "2302", "bibliographicPageStart": "2295", "bibliographicVolumeNumber": "5", "bibliographic_titles": [{}, {"bibliographic_title": "ACS Omega", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "Author", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Ripa, Rosa"}], "nameIdentifiers": [{"nameIdentifier": "8945", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shen, Amy Q."}], "nameIdentifiers": [{"nameIdentifier": "8946", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Funari, Riccardo"}], "nameIdentifiers": [{"nameIdentifier": "8947", "nameIdentifierScheme": "WEKO"}]}]}, "item_10001_description_5": {"attribute_name": "Abstract", "attribute_value_mlt": [{"subitem_description": "Bacterial biofilms are responsible for persistent infections and biofouling, raising serious concerns in both medical and industrial processes. These motivations underpin the need to develop methodologies to study the complex biological structures of biofilms and prevent their formation on medical implants, tools, and industrial apparatuses. Here, we report the detailed comparison of Escherichia coli biofilm development stages (adhesion, maturation, and dispersion) on gold and titanium surfaces by monitoring the changes in both frequency and dissipation of a quartz crystal microbalance (QCM) device, a cheap and reliable microgravimetric sensor which allows the real-time and label-free characterization of various stages of biofilm development. Although gold is the most common electrode material used for QCM sensors, the titanium electrode is also readily available for QCM sensors; thus, QCM sensors with different metal electrodes serve as a simple platform to probe how pathogens interact with different metal substrates. The QCM outcomes are further confirmed by atomic force microscopy and crystal violet staining, thus validating the effectiveness of this surface sensitive sensor for microbial biofilm research. Moreover, because QCM technology can easily modify the substrate types and coatings, QCM sensors also provide well-controlled experimental conditions to study antimicrobial surface treatments and eradication procedures, even on mature biofilms.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_publisher": "American Chemical Society"}]}, "item_10001_relation_13": {"attribute_name": "PubMedNo.", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:pmid/32064391", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1021/acsomega.9b03540", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_16": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "https://pubs.acs.org/page/policy/authorchoice_termsofuse.html"}]}]}, "item_10001_relation_17": {"attribute_name": "Related site", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://pubs.acs.org/doi/full/10.1021/acsomega.9b03540", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_rights": "© 2020 American Chemical Society"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2470-1343", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "Author\u0027s flag", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-05-12"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Ripa-2020-Detecting Escherichia coli Biofilm D.pdf", "filesize": [{"value": "6.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "ACS AuthorChoice\n(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html)", "licensetype": "license_free", "mimetype": "application/pdf", "size": 6300000.0, "url": {"label": "Ripa-2020-Detecting Escherichia coli Biofilm D", "url": "https://oist.repo.nii.ac.jp/record/1479/files/Ripa-2020-Detecting Escherichia coli Biofilm D.pdf"}, "version_id": "1c514dab-2032-44dc-bc7d-b65dd008641f"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Detecting Escherichia coli Biofilm Development Stages on Gold and Titanium by Quartz Crystal Microbalance", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Detecting Escherichia coli Biofilm Development Stages on Gold and Titanium by Quartz Crystal Microbalance", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "28", "path": ["54"], "permalink_uri": "https://oist.repo.nii.ac.jp/records/1479", "pubdate": {"attribute_name": "公開日", "attribute_value": "2020-05-12"}, "publish_date": "2020-05-12", "publish_status": "0", "recid": "1479", "relation": {}, "relation_version_is_last": true, "title": ["Detecting Escherichia coli Biofilm Development Stages on Gold and Titanium by Quartz Crystal Microbalance"], "weko_shared_id": 28}
Detecting Escherichia coli Biofilm Development Stages on Gold and Titanium by Quartz Crystal Microbalance
https://oist.repo.nii.ac.jp/records/1479
https://oist.repo.nii.ac.jp/records/1479d9118463-bd53-4dbb-b49b-945db5632130
名前 / ファイル | ライセンス | アクション |
---|---|---|
Ripa-2020-Detecting Escherichia coli Biofilm D (6.3 MB)
|
ACS AuthorChoice
(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2020-05-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Detecting Escherichia coli Biofilm Development Stages on Gold and Titanium by Quartz Crystal Microbalance | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Ripa, Rosa
× Ripa, Rosa× Shen, Amy Q.× Funari, Riccardo |
|||||
書誌情報 |
en : ACS Omega 巻 5, 号 5, p. 2295-2302, 発行日 2020-01-28 |
|||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Bacterial biofilms are responsible for persistent infections and biofouling, raising serious concerns in both medical and industrial processes. These motivations underpin the need to develop methodologies to study the complex biological structures of biofilms and prevent their formation on medical implants, tools, and industrial apparatuses. Here, we report the detailed comparison of Escherichia coli biofilm development stages (adhesion, maturation, and dispersion) on gold and titanium surfaces by monitoring the changes in both frequency and dissipation of a quartz crystal microbalance (QCM) device, a cheap and reliable microgravimetric sensor which allows the real-time and label-free characterization of various stages of biofilm development. Although gold is the most common electrode material used for QCM sensors, the titanium electrode is also readily available for QCM sensors; thus, QCM sensors with different metal electrodes serve as a simple platform to probe how pathogens interact with different metal substrates. The QCM outcomes are further confirmed by atomic force microscopy and crystal violet staining, thus validating the effectiveness of this surface sensitive sensor for microbial biofilm research. Moreover, because QCM technology can easily modify the substrate types and coatings, QCM sensors also provide well-controlled experimental conditions to study antimicrobial surface treatments and eradication procedures, even on mature biofilms. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2470-1343 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32064391 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acsomega.9b03540 | |||||
権利 | ||||||
権利情報 | © 2020 American Chemical Society | |||||
情報源 | ||||||
関連名称 | https://pubs.acs.org/page/policy/authorchoice_termsofuse.html | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/full/10.1021/acsomega.9b03540 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |