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Dynamic actin-mediated nano-scale clustering of CD44 regulates its meso-scale organization at the plasma membrane
https://oist.repo.nii.ac.jp/records/1500
https://oist.repo.nii.ac.jp/records/1500571eb507-a556-46ee-b89d-3a8069f4723f
名前 / ファイル | ライセンス | アクション |
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mbc.e18-11-0715 (4.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-05-14 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Dynamic actin-mediated nano-scale clustering of CD44 regulates its meso-scale organization at the plasma membrane | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Sil, Parijat
× Sil, Parijat× Mateos, Nicolas× Nath, Sangeeta× Buschow, Sonja× Manzo, Carlo× Suzuki, Kenichi G. N.× Fujiwara, Takahiro× Kusumi, Akihiro× Garcia-Parajo, Maria F.× Mayor, Satyajit |
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書誌情報 |
en : Molecular Biology of the Cell 巻 31, 号 7, p. 561-579, 発行日 2020-03-19 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Transmembrane adhesion receptors at the cell surface, such as CD44, are often equipped with modules to interact with the extracellular matrix (ECM) and the intracellular cytoskeletal machinery. CD44 has been recently shown to compartmentalize the membrane into domains by acting as membrane pickets, facilitating the function of signaling receptors. While spatial organization and diffusion studies of membrane proteins are usually conducted separately, here we combine observations of organization and diffusion by using high spatio-temporal resolution imaging on living cells to reveal a hierarchical organization of CD44. CD44 is present in a meso-scale meshwork pattern where it exhibits enhanced confinement and is enriched in nanoclusters of CD44 along its boundaries. This nanoclustering is orchestrated by the underlying cortical actin dynamics. Interaction with actin is mediated by specific segments of the intracellular domain. This influences the organization of the protein at the nano-scale, generating a selective requirement for formin over Arp2/3-based actin-nucleation machinery. The extracellular domain and its interaction with elements of ECM do not influence the meso-scale organization, but may serve to reposition the meshwork with respect to the ECM. Taken together, our results capture the hierarchical nature of CD44 organization at the cell surface, with active cytoskeleton-templated nanoclusters localized to a meso-scale meshwork pattern. | |||||
出版者 | ||||||
出版者 | The American Society for Cell Biology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1059-1524 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1939-4586 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/31577524 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1091/mbc.E18-11-0715 | |||||
権利 | ||||||
権利情報 | © 2020 Sil, Mateos, et al. | |||||
情報源 | ||||||
関連名称 | https://creativecommons.org/licenses/by-nc-sa/3.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.molbiolcell.org/doi/10.1091/mbc.E18-11-0715 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |