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Cryo-EM Structures of Centromeric Tri-nucleosomes Containing a Central CENP-A Nucleosome
https://oist.repo.nii.ac.jp/records/1505
https://oist.repo.nii.ac.jp/records/15051e5aa05a-bde2-4ae5-b985-fb9185f7ccaa
名前 / ファイル | ライセンス | アクション |
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Takizawa,Structure,main_text_102119_final (534.2 kB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)
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Takizawa,Structure,Supplemental_Figure (768.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-05-15 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cryo-EM Structures of Centromeric Tri-nucleosomes Containing a Central CENP-A Nucleosome | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | centromere | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | CENP-A chromatin | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cryo-EM | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | nucleosome | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | histone variant | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Takizawa, Yoshimasa
× Takizawa, Yoshimasa× Ho, Cheng-Han× Tachiwana, Hiroaki× Matsunami, Hideyuki× Kobayashi, Wataru× Suzuki, Midori× Arimura, Yasuhiro× Hori, Tetsuya× Fukagawa, Tatsuo× Ohi, Melanie D.× Wolf, Matthias× Kurumizaka, Hitoshi |
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書誌情報 |
en : Structure 巻 28, 号 1, p. 44-53.e4, 発行日 2019-11-08 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The histone H3 variant CENP-A is a crucial epigenetic marker for centromere specification. CENP-A forms a characteristic nucleosome and dictates the higher-order configuration of centromeric chromatin. However, little is known about how the CENP-A nucleosome affects the architecture of centromeric chromatin. In this study, we reconstituted tri-nucleosomes mimicking a centromeric nucleosome arrangement containing the CENP-A nucleosome, and determined their 3D structures by cryoelectron microscopy. The H3-CENP-A-H3 tri-nucleosomes adopt an untwisted architecture, with an outward-facing linker DNA path between nucleosomes. This is distinct from the H3-H3-H3 tri-nucleosome architecture, with an inward-facing DNA path. Intriguingly, the untwisted architecture may allow the CENP-A nucleosome to be exposed to the solvent in the condensed chromatin model. These results provide a structural basis for understanding the 3D configuration of CENP-A-containing chromatin, and may explain how centromeric proteins can specifically target the CENP-A nucleosomes buried in robust amounts of H3 nucleosomes in centromeres. | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0969-2126 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1878-4186 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/31711756 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.str.2019.10.016 | |||||
権利 | ||||||
権利情報 | This article/chapter was published in Structure, Volume 28, Issue 1, Yoshimasa Takizawa, Cheng-Han Ho, Hiroaki Tachiwana, Hideyuki Matsunami, Wataru Kobayashi, Midori Suzuki, Yasuhiro Arimura, Tetsuya Hori, Tatsuo Fukagawa, Melanie D. Ohi, Matthias Wolf, Hitoshi Kurumizaka, Cryo-EM Structures of Centromeric Tri-nucleosomes Containing a Central CENP-A Nucleosome, 44-53.e4, © 2019 Elsevier Ltd. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S0969212619303570 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |