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Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy
https://oist.repo.nii.ac.jp/records/1542
https://oist.repo.nii.ac.jp/records/1542a96c113c-4fe6-4068-a5c2-0e4af4387f8e
名前 / ファイル | ライセンス | アクション |
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Kuhn and Roome - Voltage Imaging with ANNINE d (1.4 MB)
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Item type | 図書の一部 / Book(1) | |||||
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公開日 | 2020-06-11 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_2f33 | |||||
資源タイプ | book | |||||
図書名 | ||||||
収録物名 | Multiphoton Microscopy | |||||
著者 |
Roome, Christopher J.
× Roome, Christopher J.× Kuhn, Bernd |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Voltage imaging is a tried and tested tool for revealing changes in neuronal membrane voltage at high temporal and spatial resolution in vitro and in vivo. However, single-photon in vivo voltage imaging using cameras and synthetic dyes does not allow depth resolution in highly scattering tissue such as the mammalian brain and risks introducing artifacts due to phototoxicity and bleaching. In contrast, voltage imaging with synthetic electrochromic dyes and two-photon excitation near the red spectral edge of absorption circumvents these challenges, allowing depth-resolved measurement of voltage changes at high spatial and temporal resolution in scattering tissue with negligible phototoxicity and bleaching. Here, we describe how to image voltage using two-photon microscopy and the voltage-sensitive dyes ANNINE-6 and ANNINE-6plus. The key advantages of these dyes are that the voltage response is linear, the temporal resolution is essentially limited by the imaging speed, and phototoxicity and bleaching can be neglected when an excitation wavelength close to the red spectral edge of absorption is chosen. We report how to image membrane voltage of dissociated cells in culture. We provide protocols for imaging average membrane voltage in vitro (in brain slices) and in anesthetized and awake animals. Finally, we describe the labeling of single neurons in vivo and how to measure supra- and sub-threshold voltage changes in their dendrites in the awake animal. Voltage can be imaged from internally labeled cells for at least 2 weeks after a single electroporation. Dendritic voltage imaging can be combined with electrical recording, calcium imaging, and/or pharmacology. |
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出版者 | ||||||
出版者 | Humana Press | |||||
開始ページ | ||||||
開始ページ | 297 | |||||
終了ページ | ||||||
終了ページ | 334 | |||||
出版年月日 | ||||||
日付 | 2019-11-01 | |||||
日付タイプ | Issued | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0893-2336 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1940-6045 | |||||
ISBN | ||||||
関連タイプ | isPartOf | |||||
識別子タイプ | ISBN | |||||
関連識別子 | 978-1-4939-9701-5 | |||||
ISBN | ||||||
関連タイプ | isPartOf | |||||
識別子タイプ | ISBN | |||||
関連識別子 | 978-1-4939-9702-2 | |||||
権利 | ||||||
権利情報 | © 2019 Springer Science+Business Media, LLC, part of Springer Nature | |||||
情報源 | ||||||
関連名称 | Springer Protocols. Neuromethods, 148. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://link.springer.com/protocol/10.1007%2F978-1-4939-9702-2_13 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |