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Interfacing with Neural Activity via Femtosecond Laser Stimulation of Drug-Encapsulating Liposomal Nanostructures
https://oist.repo.nii.ac.jp/records/1544
https://oist.repo.nii.ac.jp/records/1544253ed401-c8c4-4f3d-b640-b8f0ebd9766e
名前 / ファイル | ライセンス | アクション |
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ENEURO.0107-16.2016.full (2.0 MB)
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Creative Commons Attribution 4.0 International
(http://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-06-12 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Interfacing with Neural Activity via Femtosecond Laser Stimulation of Drug-Encapsulating Liposomal Nanostructures | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Nakano, Takashi
× Nakano, Takashi× Mackay, Sean M.× Wui Tan, Eng× Dani, Keshav M.× Wickens, Jeff |
|||||
書誌情報 |
en : eNeuro 巻 3, 号 6, p. ENEURO.0107-16.2016, 発行日 2016-11-03 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | External control over rapid and precise release of chemicals in the brain potentially provides a powerful interface with neural activity. Optical manipulation techniques, such as optogenetics and caged compounds, enable remote control of neural activity and behavior with fine spatiotemporal resolution. However, these methods are limited to chemicals that are naturally present in the brain or chemically suitable for caging. Here, we demonstrate the ability to interface with neural functioning via a wide range of neurochemicals released by stimulating loaded liposomal nanostructures with femtosecond lasers. Using a commercial two-photon microscope, we released inhibitory or excitatory neurochemicals to evoke subthreshold and suprathreshold changes in membrane potential in a live mouse brain slice. The responses were repeatable and could be controlled by adjusting laser stimulation characteristics. We also demonstrate the release of a wider range of chemicals—which previously were impossible to release by optogenetics or uncaging—including synthetic analogs of naturally occurring neurochemicals. In particular, we demonstrate the release of a synthetic receptor-specific agonist that exerts physiological effects on long-term synaptic plasticity. Further, we show that the loaded liposomal nanostructures remain functional for weeks in a live mouse. In conclusion, we demonstrate new techniques capable of interfacing with live neurons, and extendable to in vivo applications. | |||||
出版者 | ||||||
出版者 | Society for Neuroscience | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2373-2822 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/27896311 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1523/ENEURO.0107-16.2016 | |||||
権利 | ||||||
権利情報 | © 2016 Nakano et al. | |||||
情報源 | ||||||
関連名称 | http://creativecommons.org/licenses/by/4.0/ | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.eneuro.org/content/3/6/ENEURO.0107-16.2016 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |