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Metabolic engineering of a novel strain of electrogenic bacterium Arcobacter butzleri to create a platform for single analyte detection using a microbial fuel cell
https://oist.repo.nii.ac.jp/records/1707
https://oist.repo.nii.ac.jp/records/170732bfef6c-a581-4a61-a24f-c74b08a0ad68
名前 / ファイル | ライセンス | アクション |
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1-s2.0-S0141022920300570-main (1.2 MB)
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Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-10-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Metabolic engineering of a novel strain of electrogenic bacterium Arcobacter butzleri to create a platform for single analyte detection using a microbial fuel cell | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Biosensor | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | CRISPR | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Electrode associated bacteria | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Electron transfer | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Microbial fuel cell | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Szydlowski, Lukasz
× Szydlowski, Lukasz× Lan, Tammy C.T.× Shibata, Noriko× Goryanin, Igor |
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書誌情報 |
en : Enzyme and Microbial Technology 巻 139, p. 109564, 発行日 2020-04-23 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Electrogenic bacteria metabolize organic substrates by transferring electrons to the external electrode, with subsequent electricity generation. In this proof-of-concept study, we present a novel strain of a known, electrogenic Arcobacter butzleri that can grow primarily on acetate and lactate and its electric current density is positively correlated (R(2)=0.95) to the COD concentrations up to 200ppm. Using CRISPR-Cas9 and Cpf1, we engineered knockout Arcobacter butzleri mutants in either the acetate or lactate metabolic pathway, limiting their energy metabolism to a single carbon source. After genome editing, the expression of either acetate kinase, ackA, or lactate permease, lctP, was inhibited, as indicated by qPCR results. All mutants retain electrogenic activity when inoculated into a microbial fuel cell, yielding average current densities of 81-82mA/m(2), with wild type controls reaching 85-87mA(2). In the case of mutants, however, current is only generated in the presence of the substrate for the remaining pathway. Thus, we demonstrate that it is possible to obtain electric signal corresponding to the specific organic compound via genome editing. The outcome of this study also indicates that the application of electrogenic bacteria can be expanded by genome engineering. | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0141-0229 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32732044 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.enzmictec.2020.109564 | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S0141022920300570 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |