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Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel
https://oist.repo.nii.ac.jp/records/1793
https://oist.repo.nii.ac.jp/records/1793f353a171-6133-4282-9d95-38d230bbde77
名前 / ファイル | ライセンス | アクション |
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Lee-2020-Cryo-EM structure of human Cx31.3_GJC (1.6 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-11-17 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Lee, Hyuk-Joon
× Lee, Hyuk-Joon× Jeong, Hyeongseop× Hyun, Jaekyung× Ryu, Bumhan× Park, Kunwoong× Lim, Hyun-Ho× Yoo, Jejoong× Woo, Jae-Sung |
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書誌情報 |
en : Science Advances 巻 6, 号 35, p. eaba4996, 発行日 2020-08-28 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Connexin family proteins assemble into hexameric channels called hemichannels/connexons, which function as transmembrane channels or dock together to form gap junction intercellular channels (GJIChs). We determined the cryo-electron microscopy structures of human connexin 31.3 (Cx31.3)/GJC3 hemichannels in the presence and absence of calcium ions and with a hearing-loss mutation R15G at 2.3-, 2.5-, and 2.6-A resolutions, respectively. Compared with available structures of GJICh in open conformation, Cx31.3 hemichannel shows substantial structural changes of highly conserved regions in the connexin family, including opening of calcium ion-binding tunnels, reorganization of salt-bridge networks, exposure of lipid-binding sites, and collocation of amino-terminal helices at the cytoplasmic entrance. We also found that the hemichannel has a pore with a diameter of ~8 A and selectively transports chloride ions. Our study provides structural insights into the permeant selectivity of Cx31.3 hemichannel. | |||||
出版者 | ||||||
出版者 | American Association for the Advancement of Science | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2375-2548 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/32923625 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1126/sciadv.aba4996 | |||||
権利 | ||||||
権利情報 | © 2020 The Author(s), some rights reserved; exclusive licensee American Association for the Advancement of Science. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://advances.sciencemag.org/content/6/35/eaba4996 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |