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Hidden proteome of synaptic vesicles in the mammalian brain
https://oist.repo.nii.ac.jp/records/2014
https://oist.repo.nii.ac.jp/records/20149740b00e-00bd-45b0-8f2b-91f967dcb8f7
名前 / ファイル | ライセンス | アクション |
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33586.full (2.8 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-04-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Hidden proteome of synaptic vesicles in the mammalian brain | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | synapse | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | deep proteomics | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | synaptic vesicles | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | brain disorders | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | neurotransmission | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Taoufiq, Zacharie
× Taoufiq, Zacharie× Ninov, Momchil× Villar-Briones, Alejandro× Wang, Han-Ying× Sasaki, Toshio× Roy, Michael C.× Beauchain, Francois× Mori, Yasunori× Yoshida, Tomofumi× Takamori, Shigeo× Jahn, Reinhard× Takahashi, Tomoyuki |
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書誌情報 |
en : Proceedings of the National Academy of Sciences 巻 117, 号 52, p. 33586-33596, 発行日 2020-12-21 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Current proteomic studies clarified canonical synaptic proteins that are common to many types of synapses. However, proteins of diversified functions in a subset of synapses are largely hidden because of their low abundance or structural similarities to abundant proteins. To overcome this limitation, we have developed an “ultra-definition” (UD) subcellular proteomic workflow. Using purified synaptic vesicle (SV) fraction from rat brain, we identified 1,466 proteins, three times more than reported previously. This refined proteome includes all canonical SV proteins, as well as numerous proteins of low abundance, many of which were hitherto undetected. Comparison of UD quantifications between SV and synaptosomal fractions has enabled us to distinguish SV-resident proteins from potential SV-visitor proteins. We found 134 SV residents, of which 86 are present in an average copy number per SV of less than one, including vesicular transporters of nonubiquitous neurotransmitters in the brain. We provide a fully annotated resource of all categorized SV-resident and potential SV-visitor proteins, which can be utilized to drive novel functional studies, as we characterized here Aak1 as a regulator of synaptic transmission. Moreover, proteins in the SV fraction are associated with more than 200 distinct brain diseases. Remarkably, a majority of these proteins was found in the low-abundance proteome range, highlighting its pathological significance. Our deep SV proteome will provide a fundamental resource for a variety of future investigations on the function of synapses in health and disease. | |||||
出版者 | ||||||
出版者 | National Academy of Sciences | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0027-8424 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1091-6490 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/33376223 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1073/pnas.2011870117 | |||||
権利 | ||||||
権利情報 | © 2020 the Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.pnas.org/content/117/52/33586 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |