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Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP
https://oist.repo.nii.ac.jp/records/2032
https://oist.repo.nii.ac.jp/records/2032b76d1cbf-75a4-432b-ac77-9ca6e76da90e
名前 / ファイル | ライセンス | アクション |
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Bokelmann-2021-Point-of-care bulk testing for (1.5 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-05-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Bokelmann, Lukas
× Bokelmann, Lukas× Nickel, Olaf× Maricic, Tomislav× Pääbo, Svante× Meyer, Matthias× Borte, Stephan× Riesenberg, Stephan |
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書誌情報 |
en : Nature Communications 巻 12, 号 1, p. 1467, 発行日 2021-03-05 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Efforts to contain the spread of SARS-CoV-2 have spurred the need for reliable, rapid, and cost-effective diagnostic methods which can be applied to large numbers of people. However, current standard protocols for the detection of viral nucleic acids while sensitive, require a high level of automation and sophisticated laboratory equipment to achieve throughputs that allow whole communities to be tested on a regular basis. Here we present Cap-iLAMP (capture and improved loop-mediated isothermal amplification) which combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. In contrast to direct addition of the sample to improved LAMP (iLAMP), Cap-iLAMP prevents false positives and allows single positive samples to be detected in pools of 25 negative samples, reducing the reagent cost per test to ~1 Euro per individual. | |||||
出版者 | ||||||
出版者 | Nature portfolio | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2041-1723 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/33674580 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1038/s41467-021-21627-0 | |||||
権利 | ||||||
権利情報 | © 2021 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.nature.com/articles/s41467-021-21627-0 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |