WEKO3
アイテム
{"_buckets": {"deposit": "2f6b65a6-2db2-4cd3-9275-fbce01da5e9a"}, "_deposit": {"created_by": 30, "id": "2034", "owners": [30], "pid": {"revision_id": 0, "type": "depid", "value": "2034"}, "status": "published"}, "_oai": {"id": "oai:oist.repo.nii.ac.jp:00002034", "sets": ["46"]}, "author_link": ["13209", "13210", "13211"], "item_10001_biblio_info_7": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2021-03-25", "bibliographicIssueDateType": "Issued"}, "bibliographicPageStart": "609287", "bibliographicVolumeNumber": "8", "bibliographic_titles": [{}, {"bibliographic_title": "Frontiers in Marine Science", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "Author", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Chuang, Po-Shun"}], "nameIdentifiers": [{"nameIdentifier": "13209", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishikawa, Kota"}], "nameIdentifiers": [{"nameIdentifier": "13210", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Mitarai, Satoshi"}], "nameIdentifiers": [{"nameIdentifier": "13211", "nameIdentifierScheme": "WEKO"}]}]}, "item_10001_description_5": {"attribute_name": "Abstract", "attribute_value_mlt": [{"subitem_description": "Contemporary advances in microfluidic and molecular techniques have enabled coral studies to shift from reef and colony scales to polyp- and molecular-level investigations. Polyp bail-out provides an alternative approach to acquire solitary polyps for studies at finer scales. Although induction of polyp bail-out has been reported in several studies, polyp health after bail-out has not been investigated. In this study, we monitored morphological and genetic changes in Pocillopora acuta polyps after bail-out induced by hyperosmosis. In isosmotic conditions, over 80% of bailed-out polyps survived, of which half regenerated normal polyp morphology within 5 days, including a polarized polyp body, extended tentacles, and a distinguishable oral disk. In contrast, the remaining polyps degenerated into tissue ball-like structures that resemble multicellular aggregates reported in earlier studies. In morphologically recovered polyps, transcriptomic analysis showed that ∼87% of genes altered during bail-out induction recovered from stress status, suggesting resumption of metabolism, cell division, and immunity, while in degenerated polyps, only ∼71% of genes recovered. Quantitative polymerase chain reaction data further demonstrated that genetic recovery of energy production, cell proliferation, and immune response was achieved in morphologically recovered polyps within 3 days after bail-out, but was not fully accomplished in degenerated polyps even after 5 days. Our findings indicate that solitary polyps generated by hyperosmosis-induced bail-out can recover rapidly from physiological stress under laboratory conditions, suggesting that bailed-out polyps could be used as new models for coral research.", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_publisher": "Frontiers Media"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.3389/fmars.2021.609287", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_17": {"attribute_name": "Related site", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.frontiersin.org/articles/10.3389/fmars.2021.609287/full", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_rights": "© 2021 Chuang, Ishikawa and Mitarai."}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2296-7745", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "Author\u0027s flag", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-05-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Chuang-2021-Morphological and Genetic Recovery.pdf", "filesize": [{"value": "3.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)", "licensetype": "license_free", "mimetype": "application/pdf", "size": 3600000.0, "url": {"label": "Chuang-2021-Morphological and Genetic Recovery", "url": "https://oist.repo.nii.ac.jp/record/2034/files/Chuang-2021-Morphological and Genetic Recovery.pdf"}, "version_id": "caeeaaeb-3824-4ac2-a939-eddd6137abc2"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "polyp bail-out", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "micropropagation", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "polyp recovery", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "genetics", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "metabolism", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Morphological and Genetic Recovery of Coral Polyps After Bail-Out", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Morphological and Genetic Recovery of Coral Polyps After Bail-Out", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "30", "path": ["46"], "permalink_uri": "https://oist.repo.nii.ac.jp/records/2034", "pubdate": {"attribute_name": "公開日", "attribute_value": "2021-05-06"}, "publish_date": "2021-05-06", "publish_status": "0", "recid": "2034", "relation": {}, "relation_version_is_last": true, "title": ["Morphological and Genetic Recovery of Coral Polyps After Bail-Out"], "weko_shared_id": 30}
Morphological and Genetic Recovery of Coral Polyps After Bail-Out
https://oist.repo.nii.ac.jp/records/2034
https://oist.repo.nii.ac.jp/records/20342188909f-17c2-4983-a76d-0b12a09e4280
名前 / ファイル | ライセンス | アクション |
---|---|---|
Chuang-2021-Morphological and Genetic Recovery (3.6 MB)
|
Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2021-05-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Morphological and Genetic Recovery of Coral Polyps After Bail-Out | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | polyp bail-out | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | micropropagation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | polyp recovery | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | genetics | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | metabolism | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Chuang, Po-Shun
× Chuang, Po-Shun× Ishikawa, Kota× Mitarai, Satoshi |
|||||
書誌情報 |
en : Frontiers in Marine Science 巻 8, p. 609287, 発行日 2021-03-25 |
|||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Contemporary advances in microfluidic and molecular techniques have enabled coral studies to shift from reef and colony scales to polyp- and molecular-level investigations. Polyp bail-out provides an alternative approach to acquire solitary polyps for studies at finer scales. Although induction of polyp bail-out has been reported in several studies, polyp health after bail-out has not been investigated. In this study, we monitored morphological and genetic changes in Pocillopora acuta polyps after bail-out induced by hyperosmosis. In isosmotic conditions, over 80% of bailed-out polyps survived, of which half regenerated normal polyp morphology within 5 days, including a polarized polyp body, extended tentacles, and a distinguishable oral disk. In contrast, the remaining polyps degenerated into tissue ball-like structures that resemble multicellular aggregates reported in earlier studies. In morphologically recovered polyps, transcriptomic analysis showed that ∼87% of genes altered during bail-out induction recovered from stress status, suggesting resumption of metabolism, cell division, and immunity, while in degenerated polyps, only ∼71% of genes recovered. Quantitative polymerase chain reaction data further demonstrated that genetic recovery of energy production, cell proliferation, and immune response was achieved in morphologically recovered polyps within 3 days after bail-out, but was not fully accomplished in degenerated polyps even after 5 days. Our findings indicate that solitary polyps generated by hyperosmosis-induced bail-out can recover rapidly from physiological stress under laboratory conditions, suggesting that bailed-out polyps could be used as new models for coral research. | |||||
出版者 | ||||||
出版者 | Frontiers Media | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2296-7745 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3389/fmars.2021.609287 | |||||
権利 | ||||||
権利情報 | © 2021 Chuang, Ishikawa and Mitarai. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.frontiersin.org/articles/10.3389/fmars.2021.609287/full | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |