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Ca2+ activity maps of astrocytes tagged by axoastrocytic AAV transfer
https://oist.repo.nii.ac.jp/records/2060
https://oist.repo.nii.ac.jp/records/20601b5dc5b7-cb48-4a0d-a0d6-cacc8d220a81
名前 / ファイル | ライセンス | アクション |
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Georgiou-2022-Ca(+) activity maps of astrocyte (1.7 MB)
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CC BY 4.0
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-04-18 | |||||
タイトル | ||||||
タイトル | Ca2+ activity maps of astrocytes tagged by axoastrocytic AAV transfer | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Georgiou, Leonidas
× Georgiou, Leonidas× Echeverría, Anaí× Georgiou, Achilleas× Kuhn, Bernd |
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書誌情報 |
Science Advances 巻 8, 号 6, 発行日 2022-02-09 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Astrocytes exhibit localized Ca2+ microdomain (MD) activity thought to be actively involved in information processing in the brain. However, functional organization of Ca2+ MDs in space and time in relationship to behavior and neuronal activity is poorly understood. Here, we first show that adeno-associated virus (AAV) particles transfer anterogradely from axons to astrocytes. Then, we use this axoastrocytic AAV transfer to express genetically encoded Ca2+ indicators at high-contrast circuit specifically. In combination with two-photon microscopy and unbiased, event-based analysis, we investigated cortical astrocytes embedded in the vibrissal thalamocortical circuit. We found a wide range of Ca2+ MD signals, some of which were ultrafast (≤300 ms). Frequency and size of signals were extensively increased by locomotion but only subtly with sensory stimulation. The overlay of these signals resulted in behavior-dependent maps with characteristic Ca2+ activity hotspots, maybe representing memory engrams. These functional subdomains are stable over days, suggesting subcellular specialization. | |||||
出版者 | ||||||
出版者 | American Association for the Advancement of Science | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2375-2548 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35138891 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1126/sciadv.abe5371 | |||||
権利 | ||||||
権利情報 | © 2022 The Authors | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.science.org/doi/10.1126/sciadv.abe5371 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |