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Implications of divergence of methionine adenosyltransferase in archaea
https://oist.repo.nii.ac.jp/records/2338
https://oist.repo.nii.ac.jp/records/2338e724109f-bd23-4388-b595-269d2d8b9024
名前 / ファイル | ライセンス | アクション |
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2211-5463.13312 (1) (3.5 MB)
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CC BY 4.0
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-10-19 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Implications of divergence of methionine adenosyltransferase in archaea | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Methionine Adenosyltransferase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Enzyme evolution | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ancestral Sequence Reconstruction (ASR) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Catalytic interface | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Divergence | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Chouhan, Bhanu Pratap Singh
× Chouhan, Bhanu Pratap Singh× Gade, Madhuri× Martinez, Desirae× Toledo‐Patino, Saacnicteh× Laurino, Paola |
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書誌情報 |
en : FEBS Open Bio 発行日 2021-10-16 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Methionine adenosyltransferase (MAT) catalyzes the biosynthesis of S-adenosylmethionine from L-methionine and adenosine triphosphate. MAT enzymes are ancient, believed to share a common ancestor, and are highly conserved in all three domains of life. However, the sequences of archaeal MATs show considerable divergence compared to their bacterial and eukaryotic counterparts. Furthermore, the structural and functional significance of this sequence divergence are not well understood. In the present study, we employed structural analysis and ancestral sequence reconstruction (ASR) to investigate archaeal MAT divergence. We observed that the dimer interface containing the active site (which is usually well-conserved) diverged considerably between the bacterial/eukaryotic MATs and archaeal MAT. A detailed investigation of the available structures supports the sequence analysis outcome: the protein domains and subdomains of bacterial and eukaryotic MAT are more similar than those of archaea. Finally, we resurrected archaeal MAT ancestors. Interestingly, archaeal MAT ancestors show substrate specificity, which is lost during evolution. This observation supports the hypothesis of a common MAT ancestor for the three domains of life. In conclusion, we have demonstrated that archaeal MAT is an ideal system for studying an enzyme family that evolved differently in one domain compared to others while maintaining the same catalytic activity. | |||||
出版者 | ||||||
出版者 | John Wiley & Sons Ltd. on behalf of Federation of European Biochemical Societies | |||||
出版者 | ||||||
出版者 | FEBS Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2211-5463 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/34655277 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1002/2211-5463.13312 | |||||
権利 | ||||||
権利情報 | © 2021 The Author(s) | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://febs.onlinelibrary.wiley.com/doi/10.1002/2211-5463.13312 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |