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Microfluidic device flow field characterization around tumor spheroids with tunable necrosis produced in an optimized off-chip process
https://doi.org/10.15102/1394.00000213
https://doi.org/10.15102/1394.00000213c9cd0ac6-c977-4c3a-836d-f0325b5b1a52
名前 / ファイル | ライセンス | アクション |
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manuscript1-1 (5.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-07-02 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Microfluidic device flow field characterization around tumor spheroids with tunable necrosis produced in an optimized off-chip process | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
その他(別言語等)のタイトル | ||||||
著者(英) |
Baye, James
× Baye, James× Galvin, Casey× Shen, Amy Q. |
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書誌情報 |
en : Biomedical Microdevices 巻 19, 号 3, p. 59, 発行日 2017-06-30 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Tumor spheroids are a 3-D tumor model that holds promise for testing cancer therapies in vitro using microfluidic devices. Tailoring the properties of a tumor spheroid is critical for evaluating therapies over a broad range of possible indications. Using human colon cancer cells (HCT-116), we demonstrate controlled tumor spheroid growth rates by varying the number of cells initially seeded into microwell chambers. The presence of a necrotic core in the spheroids could be controlled by changing the glucose concentration of the incubation medium. This manipulation had no effect on the size of the tumor spheroids or hypoxia in the spheroid core, which has been predicted by a mathematical model in computer simulations of spheroid growth. Control over the presence of a necrotic core while maintaining other physical parameters of the spheroid presents an opportunity to assess the impact of core necrosis on therapy efficacy. Using micro-particle imaging velocimetry (micro-PIV), we characterize the hydrodynamics and mass transport of nanoparticles in tumor spheroids in a microfluidic device. We observe a geometrical dependence on the flow rate experienced by the tumor spheroid in the device, such that the “front” of the spheroid experiences a higher flow velocity than the “back” of the spheroid. Using fluorescent nanoparticles, we demonstrate a heterogeneous accumulation of nanoparticles at the tumor interface that correlates with the observed flow velocities. The penetration depth of these nanoparticles into the tumor spheroid depends on nanoparticle diameter, consistent with reports in the literature. | |||||
出版者 | ||||||
出版者 | Springer US | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1387-2176 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1572-8781 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/28667400 | |||||
権利 | ||||||
権利情報 | © 2017 Springer Science+Business Media, LLC | |||||
権利 | ||||||
権利情報 | This is a post-peer-review, pre-copyedit version of an article published in Biomedical Microdevices. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10544-017-0200-5. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://link.springer.com/article/10.1007%2Fs10544-017-0200-5 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |