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Novel Mitochondrial DNA Markers for Scleractinian Corals and Generic-Level Environmental DNA Metabarcoding
https://oist.repo.nii.ac.jp/records/2430
https://oist.repo.nii.ac.jp/records/243081bba734-b201-4856-b5a2-cab55bb55295
名前 / ファイル | ライセンス | アクション |
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fmars-08-758207 (5.6 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-01-05 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Novel Mitochondrial DNA Markers for Scleractinian Corals and Generic-Level Environmental DNA Metabarcoding | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | scleractinia | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | mitochondrial genome | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | primer development | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | metabarcoding analyses | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | environmental DNA (eDNA) | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Shinzato, Chuya
× Shinzato, Chuya× Narisoko, Haruhi× Nishitsuji, Koki× Nagata, Tomofumi× Satoh, Noriyuki× Inoue, Jun |
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書誌情報 |
en : Frontiers in Marine Science 巻 8, p. 758207, 発行日 2021-11-30 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Coral reefs, the most biodiverse habitats in the ocean, are formed by anthozoan cnidarians, the scleractinian corals. Recently, however, ongoing climate change has imperiled scleractinian corals and coral reef environments are changing drastically. Thus, convenient, high-density monitoring of scleractinian corals is essential to understand changes in coral reef communities. Environmental DNA (eDNA) metabarcoding is potentially one of the most effective means of achieving it. Using publicly available scleractinian mitochondrial genomes, we developed high-specificity primers to amplify mitochondrial 12S ribosomal RNA (12S) and cytochrome oxidase-1 (CO1) genes of diverse scleractinian corals, which could be used for genus-level metabarcoding analyses, using next-generation sequencing technologies. To confirm the effectiveness of these primers, PCR amplicon sequencing was performed using eDNA isolated along the seashore of Okinawa, Japan. We successfully amplified all eDNA samples using PCR. Approximately 93 and 72% of PCR amplicon sequences of 12S and CO1 primers originated from scleractinian 12S and CO1 genes, respectively, confirming higher specificities for coral mitochondrial genes than primers previously used for coral eDNA metabarcoding. We also found that hierarchical clustering, based on the percentage of mapped reads to each scleractinian genus, discriminates between sampling locations, suggesting that eDNA surveys are sufficiently powerful to reveal differences between coral communities separated by <1 km. We conclude that the method reported here is a powerful tool for conducting efficient eDNA surveys targeting scleractinian corals. | |||||
出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2296-7745 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3389/fmars.2021.758207 | |||||
権利 | ||||||
権利情報 | © 2021 Shinzato, Narisoko, Nishitsuji, Nagata, Satoh and Inoue. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.frontiersin.org/articles/10.3389/fmars.2021.758207/full | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |