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Chromatin-contact atlas reveals disorder-mediated protein interactions and moonlighting chromatin-associated RBPs
https://oist.repo.nii.ac.jp/records/2541
https://oist.repo.nii.ac.jp/records/2541ea173221-be77-470e-ae43-2bae760d9e8d
名前 / ファイル | ライセンス | アクション |
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gkab1180 (4.0 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-02-03 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Chromatin-contact atlas reveals disorder-mediated protein interactions and moonlighting chromatin-associated RBPs | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Rafiee, Mahmoud-Reza
× Rafiee, Mahmoud-Reza× Zagalak, Julian A× Sidorov, Sviatoslav× Steinhauser, Sebastian× Davey, Karen× Ule, Jernej× Luscombe, Nicholas M |
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書誌情報 |
en : Nucleic Acids Research 巻 49, 号 22, p. 13092-13107, 発行日 2021-12-06 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | RNA-binding proteins (RBPs) play diverse roles in regulating co-transcriptional RNA-processing and chromatin functions, but our knowledge of the repertoire of chromatin-associated RBPs (caRBPs) and their interactions with chromatin remains limited. Here, we developed SPACE (Silica Particle Assisted Chromatin Enrichment) to isolate global and regional chromatin components with high specificity and sensitivity, and SPACEmap to identify the chromatin-contact regions in proteins. Applied to mouse embryonic stem cells, SPACE identified 1459 chromatin-associated proteins, ∼48% of which are annotated as RBPs, indicating their dual roles in chromatin and RNA-binding. Additionally, SPACEmap stringently verified chromatin-binding of 403 RBPs and identified their chromatin-contact regions. Notably, SPACEmap showed that about 40% of the caRBPs bind chromatin by intrinsically disordered regions (IDRs). Studying SPACE and total proteome dynamics from mES cells grown in 2iL and serum medium indicates significant correlation (R = 0.62). One of the most dynamic caRBPs is Dazl, which we find co-localized with PRC2 at transcription start sites of genes that are distinct from Dazl mRNA binding. Dazl and other PRC2-colocalised caRBPs are rich in intrinsically disordered regions (IDRs), which could contribute to the formation and regulation of phase-separated PRC condensates. Together, our approach provides an unprecedented insight into IDR-mediated interactions and caRBPs with moonlighting functions in native chromatin. | |||||
出版者 | ||||||
出版者 | Oxford University Press on behalf of Nucleic Acids Research. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0305-1048 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1362-4962 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/34871434 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1093/nar/gkab1180 | |||||
権利 | ||||||
権利情報 | © 2021 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://academic.oup.com/nar/article/49/22/13092/6454268?login=true | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |