WEKO3
アイテム
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Assessment of Possible Contributions of Hyaluronan and Proteoglycan Binding Link Protein 4 to Differential Perineuronal Net Formation at the Calyx of Held
https://oist.repo.nii.ac.jp/records/2575
https://oist.repo.nii.ac.jp/records/2575e124ddc6-205d-43a2-b9cb-03da87684df0
名前 / ファイル | ライセンス | アクション |
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fcell-09-730550 (6.0 MB)
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Creative Commons Attribution 4.0 International(https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-02-28 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Assessment of Possible Contributions of Hyaluronan and Proteoglycan Binding Link Protein 4 to Differential Perineuronal Net Formation at the Calyx of Held | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | perineuronal net | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | chondroitin sulfate proteoglycan | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | calyx of Held | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | hyaluronan and proteoglycan binding link protein 4 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | synapse | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | in situ proximity ligation assay | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Nojima, Kojiro
× Nojima, Kojiro× Miyazaki, Haruko× Hori, Tetsuya× Vargova, Lydia× Oohashi, Toshitaka |
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書誌情報 |
en : Frontiers in Cell and Developmental Biology 巻 9, p. 730550, 発行日 2021-09-17 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The calyx of Held is a giant nerve terminal mediating high-frequency excitatory input to principal cells of the medial nucleus of the trapezoid body (MNTB). MNTB principal neurons are enwrapped by densely organized extracellular matrix structures, known as perineuronal nets (PNNs). Emerging evidence indicates the importance of PNNs in synaptic transmission at the calyx of Held. Previously, a unique differential expression of aggrecan and brevican has been reported at this calyceal synapse. However, the role of hyaluronan and proteoglycan binding link proteins (HAPLNs) in PNN formation and synaptic transmission at this synapse remains elusive. This study aimed to assess immunohistochemical evidence for the effect of HAPLN4 on differential PNN formation at the calyx of Held. Genetic deletion of Hapln4 exhibited a clear ectopic shift of brevican localization from the perisynaptic space between the calyx of Held terminals and principal neurons to the neuropil surrounding the whole calyx of Held terminals. In contrast, aggrecan expression showed a consistent localization at the surrounding neuropil, together with HAPLN1 and tenascin-R, in both gene knockout (KO) and wild-type (WT) mice. An in situ proximity ligation assay demonstrated the molecular association of brevican with HAPLN4 in WT and HAPLN1 in gene KO mice. Further elucidation of the roles of HAPLN4 may highlight the developmental and physiological importance of PNN formation in the calyx of Held. | |||||
出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2296-634X | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/34604231 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3389/fcell.2021.730550 | |||||
権利 | ||||||
権利情報 | © 2021 Nojima, Miyazaki, Hori, Vargova and Oohashi. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.frontiersin.org/articles/10.3389/fcell.2021.730550/full | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |