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Optimization and Fabrication of Multi-Level Microchannels for Long-Term Imaging of Bacterial Growth and Expansion
https://oist.repo.nii.ac.jp/records/2640
https://oist.repo.nii.ac.jp/records/2640f49850d4-3cda-4153-8bb6-b82376a83d62
名前 / ファイル | ライセンス | アクション |
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Tsai-2022-Optimization and Fabrication of Mult (4.7 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-05-18 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Optimization and Fabrication of Multi-Level Microchannels for Long-Term Imaging of Bacterial Growth and Expansion | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | multi-level microfluidic device|live cell imaging|long-term microscopy imaging|focus drifting|immersion oil viscosity|bacterial population dynamics|single-cell studies|E. coli|mother machine|computational fluid dynamics | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者(英) |
Tsai, Hsieh-Fu
× Tsai, Hsieh-Fu× Carlson, Daniel× Koldaeva, Anzhelika× Pigolotti, Simone× Shen, Amy |
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書誌情報 |
en : Micromachines 巻 13, 号 4, p. 576, 発行日 2022-04-07 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Bacteria are unicellular organisms whose length is usually around a few micrometers. Advances in microfabrication techniques have enabled the design and implementation of microdevices to confine and observe bacterial colony growth. Microstructures hosting the bacteria and microchannels for nutrient perfusion usually require separate microfabrication procedures due to different feature size requirements. This fact increases the complexity of device integration and assembly process. Furthermore, long-term imaging of bacterial dynamics over tens of hours requires stability in the microscope focusing mechanism to ensure less than one-micron drift in the focal axis. In this work, we design and fabricate an integrated multi-level, hydrodynamically-optimized microfluidic chip to study long-term Escherichia coli population dynamics in confined microchannels. Reliable long-term microscopy imaging and analysis has been limited by focus drifting and ghost effect, probably caused by the shear viscosity changes of aging microscopy immersion oil. By selecting a microscopy immersion oil with the most stable viscosity, we demonstrate successful captures of focally stable time-lapse bacterial images for ≥72 h. Our fabrication and imaging methodology should be applicable to other single-cell studies requiring long-term imaging. | |||||
言語 | en | |||||
出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | EISSN | |||||
収録物識別子 | 2072-666X | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 35457881 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3390/mi13040576 | |||||
権利 | ||||||
権利情報 | © 2022 The Authors. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.mdpi.com/2072-666X/13/4/576/htm | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |