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Designing AAV Vectors for Monitoring the Subtle Calcium Fluctuations of Inferior Olive Network in vivo
https://oist.repo.nii.ac.jp/records/2648
https://oist.repo.nii.ac.jp/records/264892d382e8-feae-4e08-a0c6-83e963fdeee3
名前 / ファイル | ライセンス | アクション |
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fncel-16-825056 (8.4 MB)
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Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-06-03 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Designing AAV Vectors for Monitoring the Subtle Calcium Fluctuations of Inferior Olive Network in vivo | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | genetic targeting | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | adeno-associated virus (AAV) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | subthreshold oscillation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | calcium imaging | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | cerebellum | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | inferior olive | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Dorgans, Kevin
× Dorgans, Kevin× Guo, Da× Kurima, Kiyoto× Wickens, Jeff× Uusisaari, Marylka Yoe |
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書誌情報 |
en : Frontiers in Cellular Neuroscience 巻 16, p. 825056, 発行日 2022-04-27 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Adeno-associated viral (AAV) vectors, used as vehicles for gene transfer into the brain, are a versatile and powerful tool of modern neuroscience that allow identifying specific neuronal populations, monitoring and modulating their activity. For consistent and reproducible results, the AAV vectors must be engineered so that they reliably and accurately target cell populations. Furthermore, transgene expression must be adjusted to sufficient and safe levels compatible with the physiology of studied cells. We undertook the effort to identify and validate an AAV vector that could be utilized for researching the inferior olivary (IO) nucleus, a structure gating critical timing-related signals to the cerebellum. By means of systematic construct generation and quantitative expression profiling, we succeeded in creating a viral tool for specific and strong transfection of the IO neurons without adverse effects on their physiology. The potential of these tools is demonstrated by expressing the calcium sensor GCaMP6s in adult mouse IO neurons. We could monitor subtle calcium fluctuations underlying two signatures of intrinsic IO activity: the subthreshold oscillations (STOs) and the variable-duration action potential waveforms both in-vitro and in-vivo. Further, we show that the expression levels of GCaMP6s allowing such recordings are compatible with the delicate calcium-based dynamics of IO neurons, inviting future work into the network dynamics of the olivo-cerebellar system in behaving animals. | |||||
出版者 | ||||||
出版者 | Frontiers Media | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1662-5102 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35573836 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3389/fncel.2022.825056 | |||||
権利 | ||||||
権利情報 | © 2022 Dorgans, Guo, Kurima, Wickens and Uusisaari. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.frontiersin.org/articles/10.3389/fncel.2022.825056/full | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |