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Development of DNA markers that distinguish male and female haploid germlings of the brown alga, Cladosiphon okamuranus
https://oist.repo.nii.ac.jp/records/2664
https://oist.repo.nii.ac.jp/records/2664b0a7a83c-92e3-4fe6-aae3-570f31bc2e58
名前 / ファイル | ライセンス | アクション |
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Nishitsuji-Development of DNA markers that dis (2.2 MB)
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CC BY 4.0
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-06-20 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Development of DNA markers that distinguish male and female haploid germlings of the brown alga, Cladosiphon okamuranus | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | brown alga cultivation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cladosiphon okamuranus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | diploid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | haploid male and haploid female germlings | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | marker genes for each phase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | polymerase chain reaction primers | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Nishitsuji, Koki
× Nishitsuji, Koki× Nishitsuji, Yoshie× Yonashiro, Yoshitaka× Satoh, Noriyuki |
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書誌情報 |
en : Phycological Research 発行日 2022-06-08 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Okinawa mozuku, Cladosiphon okamuranus, is one of many edible brown algae, yielding 22 Kton annually and contributing ¥5 billion to the Japanese economy. The life cycle of C. okamuranus is complicated, since the alga has self-cloning life cycles in both haploid (N, male and female) and diploid (2N) conditions, but only diploid “seeds” (germlings) become edible sporophytes. Because haploid and diploid germlings are morphologically indistinguishable, haploid germlings are often mistakenly combined with diploid germlings for cultivation, which results in less efficient harvesting of mozuku. Sexual identification of haploid germlings is essential to develop better diploid strains by crossbreeding. With this aim, we performed RNA-seq analysis of haploid germlings of C. okamuranus. Using its decoded diploid genome and transcriptomic information, we identified 269 genes that are expressed specifically in male or female haploids. BLAST analysis with Ectocarpus siliculosus gene models revealed that nine of 269 genes were putative sex determination-related genes of C. okamuranus. A unique set of polymerase chain reaction primers for these nine genes was designed, and DNA amplification using primers enabled us to distinguish male and female haploid and diploid germlings. This tool will enable mozuku farmers to select diploid germlings free of haploid germlings. Using this DNA marker technique, the amount of mozuku cultivated in Okinawa is expected to increase. | |||||
出版者 | ||||||
出版者 | John Wiley & Sons Australia, Ltd | |||||
出版者 | ||||||
出版者 | Japanese Society of Phycology | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1322-0829 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1440-1835 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1111/pre.12489 | |||||
権利 | ||||||
権利情報 | © 2022 The Authors. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://onlinelibrary.wiley.com/doi/10.1111/pre.12489 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |