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CNOT7 Outcompetes Its Paralog CNOT8 for Integration into The CCR4-NOT Complex
https://oist.repo.nii.ac.jp/records/2696
https://oist.repo.nii.ac.jp/records/269616ea20f1-6b9f-427e-8b4e-70573162a4cf
名前 / ファイル | ライセンス | アクション |
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1-s2.0-S0022283622000973-main (3.0 MB)
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CC BY-NC-ND 4.0
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2022-07-06 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | CNOT7 Outcompetes Its Paralog CNOT8 for Integration into The CCR4-NOT Complex | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | deadenylase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | deadenylation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | mRNA stability | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | protein stability | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CNOT1 scaffold | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Stoney, Patrick N.
× Stoney, Patrick N.× Yanagiya, Akiko× Nishijima, Saori× Yamamoto, Tadashi |
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書誌情報 |
en : Journal of Molecular Biology 巻 434, 号 9, p. 167523, 発行日 2022-03-21 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The CCR4-NOT deadenylase complex is a major post-transcriptional regulator of eukaryotic gene expression. CNOT7 and CNOT8 are both vertebrate homologs of the yeast CCR4-NOT catalytic subunit Caf1. They are highly similar and are sometimes considered redundant, but Cnot7 and Cnot8 knockout mice exhibit different phenotypes, implying distinct physiological functions. In this study, we reveal a non-reciprocal effect of CNOT7 on CNOT8, in which CNOT8 protein is increased in the depletion of CNOT7 without corresponding changes in mRNA levels whereas CNOT7 is not affected by the loss of CNOT8. Cnot8 mRNA may be bound by the CCR4-NOT complex, suggesting that CCR4-NOT might directly regulate CNOT8 expression. Cnot8 mRNA is relatively unstable, but Cnot7 knockdown did not stabilize Cnot8 mRNA, nor did it increase translation. CNOT8 protein was also less stable than CNOT7. CNOT7 showed greater affinity than CNOT8 for the CCR4-NOT scaffold protein CNOT1 and was able to block CNOT8 from binding to CNOT1. Depletion of CNOT7 increased CNOT8 incorporation into the CCR4-NOT complex and stabilized CNOT8. These data suggest that CNOT7 is the dominant paralog in CCR4-NOT and that CNOT7 and CNOT8 protein stability is regulated in distinct ways. | |||||
出版者 | ||||||
出版者 | Elsevier Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0022-2836 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35248544 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1016/j.jmb.2022.167523 | |||||
権利 | ||||||
権利情報 | © 2022 The Author(s) | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.sciencedirect.com/science/article/pii/S0022283622000973 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |