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Identification of an ERN1 target site within EGFP mRNA
https://oist.repo.nii.ac.jp/records/2732
https://oist.repo.nii.ac.jp/records/2732705564b7-c32a-49f1-8a20-7d6ea0dd35bd
名前 / ファイル | ライセンス | アクション |
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Baeken-2022-Identification of an ERN1 target s (5.8 MB)
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CC BY-NC 4.0
Creative Commons Attribution-NonCommercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-08-04 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Identification of an ERN1 target site within EGFP mRNA | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | EGFP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ERN1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | IRE1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | proteostasis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | unfolded protein response | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Baeken, Marius W.
× Baeken, Marius W.× Yokobayashi, Yohei |
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書誌情報 |
en : Journal of Cellular Biochemistry 発行日 2022-07-31 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | EGFP (enhanced green fluorescent protein) is one of the most common tools used in life sciences, including research focusing on proteostasis. Here we report that ERN1 (endoplasmic reticulum to nucleus signaling 1), which is upregulated by UPR (unfolded protein response), targets an RNA hairpin loop motif in EGFP mRNA. A silent mutation introduced into EGFP mRNA abolished the ERN1-dependent mRNA decay. Therefore, experiments that employ EGFP as a reporter gene in studies that involve upregulation of the UPR pathway should be interpreted carefully, and a mutant devoid of the ERN1 target motif may be more suitable for such studies. | |||||
出版者 | ||||||
出版者 | Wiley Periodicals LLC. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0730-2312 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1097-4644 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35908204 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1002/jcb.30314 | |||||
権利 | ||||||
権利情報 | © 2022 The Authors. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://onlinelibrary.wiley.com/doi/10.1002/jcb.30314 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |