WEKO3
アイテム
{"_buckets": {"deposit": "274eefe8-0559-44cc-a054-223016654dc1"}, "_deposit": {"created_by": 33, "id": "2750", "owners": [33], "pid": {"revision_id": 0, "type": "depid", "value": "2750"}, "status": "published"}, "_oai": {"id": "oai:oist.repo.nii.ac.jp:00002750", "sets": ["2"]}, "author_link": ["17698", "17695", "17701", "17699", "17696", "17697", "17700"], "item_10001_biblio_info_7": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2022-05-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "9", "bibliographicPageStart": "5108", "bibliographicVolumeNumber": "23", "bibliographic_titles": [{}, {"bibliographic_title": "International Journal of Molecular Sciences", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "Author", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Shin, Min-Kyoung"}], "nameIdentifiers": [{"nameIdentifier": "17695", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Bang, Jin Seok"}], "nameIdentifiers": [{"nameIdentifier": "17696", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Lee, Jeoung Eun"}], "nameIdentifiers": [{"nameIdentifier": "17697", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tran, Hoang-Dai"}], "nameIdentifiers": [{"nameIdentifier": "17698", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Park, Genehong"}], "nameIdentifiers": [{"nameIdentifier": "17699", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Lee, Dong Ryul"}], "nameIdentifiers": [{"nameIdentifier": "17700", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Jo, Junghyun"}], "nameIdentifiers": [{"nameIdentifier": "17701", "nameIdentifierScheme": "WEKO"}]}]}, "item_10001_description_5": {"attribute_name": "Abstract", "attribute_value_mlt": [{"subitem_description": "In vitro organoids derived from human pluripotent stem cells (hPSCs) have been developed as essential tools to study the underlying mechanisms of human development and diseases owing to their structural and physiological similarity to corresponding organs. Despite recent advances, there are a few methodologies for three-dimensional (3D) skeletal muscle differentiation, which focus on the terminal differentiation into myofibers and investigate the potential of modeling neuromuscular disorders and muscular dystrophies. However, these methodologies cannot recapitulate the developmental processes and lack regenerative capacity. In this study, we developed a new method to differentiate hPSCs into a 3D human skeletal muscle organoid (hSkMO). This organoid model could recapitulate the myogenesis process and possesses regenerative capacities of sustainable satellite cells (SCs), which are adult muscle stem/progenitor cells capable of self-renewal and myogenic differentiation. Our 3D model demonstrated myogenesis through the sequential occurrence of multiple myogenic cell types from SCs to myocytes. Notably, we detected quiescent, non-dividing SCs throughout the hSkMO differentiation in long-term culture. They were activated and differentiated to reconstitute muscle tissue upon damage. Thus, hSkMOs can recapitulate human skeletal muscle development and regeneration and may provide a new model for studying human skeletal muscles and related diseases. ", "subitem_description_type": "Other"}]}, "item_10001_publisher_8": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_publisher": "MDPI"}]}, "item_10001_relation_13": {"attribute_name": "PubMedNo.", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:pmid/35563499", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.3390/ijms23095108", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_17": {"attribute_name": "Related site", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.mdpi.com/1422-0067/23/9/5108", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "Rights", "attribute_value_mlt": [{"subitem_rights": "© 2022 The Authors."}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1422-0067", "subitem_source_identifier_type": "ISSN"}, {"subitem_source_identifier": "1661-6596", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "Author\u0027s flag", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-08-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Shin-2022-Generation of Skeletal Muscle Organo.pdf", "filesize": [{"value": "2.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)", "licensetype": "license_free", "mimetype": "application/pdf", "size": 2200000.0, "url": {"label": "Shin-2022-Generation of Skeletal Muscle Organo", "url": "https://oist.repo.nii.ac.jp/record/2750/files/Shin-2022-Generation of Skeletal Muscle Organo.pdf"}, "version_id": "1acb1216-d2d7-41a1-9b3f-5028c88194ce"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "human pluripotent stem cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "skeletal muscle organoids", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "satellite cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "myogenesis", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "regeneration", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "33", "path": ["2"], "permalink_uri": "https://oist.repo.nii.ac.jp/records/2750", "pubdate": {"attribute_name": "公開日", "attribute_value": "2022-08-16"}, "publish_date": "2022-08-16", "publish_status": "0", "recid": "2750", "relation": {}, "relation_version_is_last": true, "title": ["Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration"], "weko_shared_id": 33}
Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration
https://oist.repo.nii.ac.jp/records/2750
https://oist.repo.nii.ac.jp/records/2750f5abdaf0-7b49-4259-87ad-db707398ee3d
名前 / ファイル | ライセンス | アクション |
---|---|---|
Shin-2022-Generation of Skeletal Muscle Organo (2.2 MB)
|
Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-08-16 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Generation of Skeletal Muscle Organoids from Human Pluripotent Stem Cells to Model Myogenesis and Muscle Regeneration | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | human pluripotent stem cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | skeletal muscle organoids | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | satellite cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | myogenesis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | regeneration | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Shin, Min-Kyoung
× Shin, Min-Kyoung× Bang, Jin Seok× Lee, Jeoung Eun× Tran, Hoang-Dai× Park, Genehong× Lee, Dong Ryul× Jo, Junghyun |
|||||
書誌情報 |
en : International Journal of Molecular Sciences 巻 23, 号 9, p. 5108, 発行日 2022-05-04 |
|||||
抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | In vitro organoids derived from human pluripotent stem cells (hPSCs) have been developed as essential tools to study the underlying mechanisms of human development and diseases owing to their structural and physiological similarity to corresponding organs. Despite recent advances, there are a few methodologies for three-dimensional (3D) skeletal muscle differentiation, which focus on the terminal differentiation into myofibers and investigate the potential of modeling neuromuscular disorders and muscular dystrophies. However, these methodologies cannot recapitulate the developmental processes and lack regenerative capacity. In this study, we developed a new method to differentiate hPSCs into a 3D human skeletal muscle organoid (hSkMO). This organoid model could recapitulate the myogenesis process and possesses regenerative capacities of sustainable satellite cells (SCs), which are adult muscle stem/progenitor cells capable of self-renewal and myogenic differentiation. Our 3D model demonstrated myogenesis through the sequential occurrence of multiple myogenic cell types from SCs to myocytes. Notably, we detected quiescent, non-dividing SCs throughout the hSkMO differentiation in long-term culture. They were activated and differentiated to reconstitute muscle tissue upon damage. Thus, hSkMOs can recapitulate human skeletal muscle development and regeneration and may provide a new model for studying human skeletal muscles and related diseases. | |||||
出版者 | ||||||
出版者 | MDPI | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1422-0067 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1661-6596 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35563499 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.3390/ijms23095108 | |||||
権利 | ||||||
権利情報 | © 2022 The Authors. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.mdpi.com/1422-0067/23/9/5108 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |