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Cohesin ATPase activities regulate DNA binding and coiled-coil configuration
https://oist.repo.nii.ac.jp/records/2769
https://oist.repo.nii.ac.jp/records/2769c1994cf3-9cf6-4858-9041-0f168cd6e657
名前 / ファイル | ライセンス | アクション |
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Xu-2022-Cohesin ATPase activities regulate DNA (3.2 MB)
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CC BY-NC-ND 4.0
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International(https://creativecommons.org/licenses/by-nc-nd/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2022-08-25 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cohesin ATPase activities regulate DNA binding and coiled-coil configuration | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cohesin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ATPase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | suppressor screen | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA binding | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | coiled coil | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Xu, Xingya
× Xu, Xingya× Kanai, Ryuta× Wang, Li× Yanagida, Mitsuhiro |
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書誌情報 |
en : Proceedings of the National Academy of Sciences 巻 119, 号 33, p. e2208004119, 発行日 2022-08-08 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | The cohesin complex is required for sister chromatid cohesion and genome compaction. Cohesin coiled coils (CCs) can fold at break sites near midpoints to bring head and hinge domains, located at opposite ends of coiled coils, into proximity. Whether ATPase activities in the head play a role in this conformational change is yet to be known. Here, we dissected functions of cohesin ATPase activities in cohesin dynamics in Schizosaccharomyces pombe. Isolation and characterization of cohesin ATPase temperature-sensitive (ts) mutants indicate that both ATPase domains are required for proper chromosome segregation. Unbiased screening of spontaneous suppressor mutations rescuing the temperature lethality of cohesin ATPase mutants identified several suppressor hotspots in cohesin that located outside of ATPase domains. Then, we performed comprehensive saturation mutagenesis targeted to these suppressor hotspots. Large numbers of the identified suppressor mutations indicated several different ways to compensate for the ATPase mutants: 1) Substitutions to amino acids with smaller side chains in coiled coils at break sites around midpoints may enable folding and extension of coiled coils more easily; 2) substitutions to arginine in the DNA binding region of the head may enhance DNA binding; or 3) substitutions to hydrophobic amino acids in coiled coils, connecting the head and interacting with other subunits, may alter conformation of coiled coils close to the head. These results reflect serial structural changes in cohesin driven by its ATPase activities potentially for packaging DNAs. | |||||
出版者 | ||||||
出版者 | National Academy of Sciences | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0027-8424 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1091-6490 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/35939705 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1073/pnas.2208004119 | |||||
権利 | ||||||
権利情報 | © 2022 The Author(s) | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.pnas.org/doi/full/10.1073/pnas.2208004119 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |