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Water without windows: Evaluating the performance of open cell transmission electron microscopy under saturated water vapor conditions, and assessing its potential for microscopy of hydrated biological specimens
https://oist.repo.nii.ac.jp/records/339
https://oist.repo.nii.ac.jp/records/339cea07d72-bddb-4b32-bcb1-a2d1208f883d
名前 / ファイル | ライセンス | アクション |
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journal.pone.0186899 (30.7 MB)
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Creative Commons Attribution 4.0 International
(http://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-03-16 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Water without windows: Evaluating the performance of open cell transmission electron microscopy under saturated water vapor conditions, and assessing its potential for microscopy of hydrated biological specimens | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Cassidy, Cathal
× Cassidy, Cathal× Yamashita, Masao× Cheung, Martin× Kalale, Chola× Adaniya, Hidehito× Kuwahara, Ryusuke× Shintake, Tsumoru |
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書誌情報 |
en : PLOS ONE 巻 12, 号 11, p. e0186899, 発行日 2017-11-03 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | We have performed open cell transmission electron microscopy experiments through pure water vapor in the saturation pressure regime (>0.6 kPa), in a modern microscope capable of sub-Å resolution. We have systematically studied achievable pressure levels, stability and gas purity, effective thickness of the water vapor column and associated electron scattering processes, and the effect of gas pressure on electron optical resolution and image contrast. For example, for 1.3 kPa pure water vapor and 300kV electrons, we report pressure stability of ± 20 Pa over tens of minutes, effective thickness of 0.57 inelastic mean free paths, lattice resolution of 0.14 nm on a reference Au specimen, and no significant degradation in contrast or stability of a biological specimen (M13 virus, with 6 nm body diameter). We have also done some brief experiments to confirm feasibility of loading specimens into an in situ water vapor ambient without exposure to intermediate desiccating conditions. Finally, we have also checked if water experiments had any discernible impact on the microscope performance, and report pertinent vacuum and electron optical data, for reference purposes. | |||||
出版者 | ||||||
出版者 | PLOS | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1932-6203 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/29099843 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1371/journal.pone.0186899 | |||||
権利 | ||||||
権利情報 | © 2017 Cassidy et al. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0186899 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |