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Deep Sequencing Analysis of Aptazyme Variants Based on a Pistol Ribozyme
https://oist.repo.nii.ac.jp/records/375
https://oist.repo.nii.ac.jp/records/375eae446ab-933f-4675-b0b4-d62a02bf3314
名前 / ファイル | ライセンス | アクション |
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acssynbio.7b00057 (972.9 kB)
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ACS AuthorChoice
(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-04-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Deep Sequencing Analysis of Aptazyme Variants Based on a Pistol Ribozyme | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Kobori, Shungo
× Kobori, Shungo× Takahashi, Kei× Yokobayashi, Yohei |
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書誌情報 |
en : ACS Synthetic Biology 巻 6, 号 7, p. 1283-1288, 発行日 2017-04-11 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Chemically regulated self-cleaving ribozymes, or aptazymes, are emerging as a promising class of genetic devices that allow dynamic control of gene expression in synthetic biology. However, further expansion of the limited repertoire of ribozymes and aptamers, and development of new strategies to couple the RNA elements to engineer functional aptazymes are highly desirable for synthetic biology applications. Here, we report aptazymes based on the recently identified self-cleaving pistol ribozyme class using a guanine aptamer as the molecular sensing element. Two aptazyme architectures were studied by constructing and assaying 17 728 mutants by deep sequencing. Although one of the architectures did not yield functional aptazymes, a novel aptazyme design in which the aptamer and the ribozyme were placed in tandem yielded a number of guanine-inhibited ribozymes. Detailed analysis of the extensive sequence-function data suggests a mechanism that involves a competition between two mutually exclusive RNA structures reminiscent of natural bacterial riboswitches. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2161-5063 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/28398719 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acssynbio.7b00057 | |||||
権利 | ||||||
権利情報 | © 2017 American Chemical Society | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/10.1021/acssynbio.7b00057 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |