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Cryo-EM structure of the nucleosome containing the ALB1 enhancer DNA sequence
https://oist.repo.nii.ac.jp/records/669
https://oist.repo.nii.ac.jp/records/669b919debf-24a0-4b99-b9dc-abdfaf805e6d
名前 / ファイル | ライセンス | アクション |
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170255.full (1.3 MB)
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Creative Commons Attribution 4.0 International
(http://creativecommons.org/licenses/by/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-08-15 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Cryo-EM structure of the nucleosome containing the ALB1 enhancer DNA sequence | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Takizawa, Yoshimasa
× Takizawa, Yoshimasa× Tanaka, Hiroki× Machida, Shinichi× Koyama, Masako× Maehara, Kazumitsu× Ohkawa, Yasuyuki× Wade, Paul A.× Wolf, Matthias× Kurumizaka, Hitoshi |
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書誌情報 |
en : Open Biology 巻 8, p. 170255, 発行日 2018-03-21 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Pioneer transcription factors specifically target their recognition DNA sequences within nucleosomes. FoxA is the pioneer transcription factor that binds to the ALB1 gene enhancer in liver precursor cells, and is required for liver differentiation in embryos. The ALB1 enhancer DNA sequence is reportedly incorporated into nucleosomes in cells, although the nucleosome structure containing the targeting sites for FoxA has not been clarified yet. In this study, we determined the nucleosome structure containing the ALB1 enhancer (N1) sequence, by cryogenic electron microscopy at 4.0 Å resolution. The nucleosome structure with the ALB1 enhancer DNA is not significantly different from the previously reported nucleosome structure with the Widom 601 DNA. Interestingly, in the nucleosomes, the ALB1 enhancer DNA contains local flexible regions, as compared to the Widom 601 DNA. Consistently, DNaseI treatments revealed that, in the nucleosome, the ALB1 enhancer (N1) DNA is more accessible than the Widom 601 sequence. The histones also associated less strongly with the ALB1 enhancer (N1) DNA than the Widom 601 DNA in the nucleosome. Therefore, the local histone–DNA contacts may be responsible for the enhanced DNA accessibility in the nucleosome with the ALB1 enhancer DNA. | |||||
出版者 | ||||||
出版者 | The Royal Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2046-2441 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/29563192 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1098/rsob.170255 | |||||
権利 | ||||||
権利情報 | © 2018 The Authors. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://rsob.royalsocietypublishing.org/content/8/3/170255 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |