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Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa
https://oist.repo.nii.ac.jp/records/800
https://oist.repo.nii.ac.jp/records/8006f56399f-6f3b-4e66-8e19-c1a7e561e54f
名前 / ファイル | ライセンス | アクション |
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Manuscript レポジトリ (656.9 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-01-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Identification of DNA methylated regions by using methylated DNA immunoprecipitation sequencing in Brassica rapa | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Takahashi, Satoshi
× Takahashi, Satoshi× Fukushima, Naoki× Osabe, Kenji× Itabashi, Etsuko× Shimizu, Motoki× Miyaji, Naomi× Takasaki-Yasuda, Takeshi× Suzuki, Yutaka× Seki, Motoaki× Fujimoto, Ryo |
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書誌情報 |
en : Crop and Pasture Science 巻 69, 号 1, p. 107-120, 発行日 2018-01-04 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | DNA methylation is an epigenetic gene regulatory mechanism that plays an essential role in gene expression, transposon silencing, genome imprinting and plant development. We investigated the influence of DNA methylation on gene expression in Brassica rapa L., to understand whether epigenetic differences exist between inbred lines. Genome-wide DNA methylation was analysed by methylated DNA immunoprecipitation sequencing (MeDIP-seq) of 14-day-old first and second leaves from two inbred lines of Chinese cabbage, one susceptible and one resistant to fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans. MACS (model-based analysis for ChIP-seq) identified DNA methylation peaks in genic regions including 2 kb upstream, exon, intron and 2 kb downstream. More than 65% of genes showed similar patterns of DNA methylation in the genic regions in the two inbred lines. DNA methylation states of the two inbred lines were compared with their transcriptome. Genes having DNA methylation in the intron and in the 200 bp upstream and downstream regions were associated with a lower expression level in both lines. A small number of genes showed a negative correlation between differences in DNA methylation levels and differences in transcriptional levels in the two inbred lines, suggesting that DNA methylation in these genes results in transcriptional suppression. | |||||
出版者 | ||||||
出版者 | CSIRO Publishing | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1836-0947 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1836-5795 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1071/CP17394 | |||||
権利 | ||||||
権利情報 | ©2018 CSIRO Publishing | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.publish.csiro.au/cp/CP17394 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |