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Genome-wide characterization of DNA methylation, small RNA expression, and histone H3 lysine nine di-methylation in Brassica rapa L.
https://oist.repo.nii.ac.jp/records/812
https://oist.repo.nii.ac.jp/records/8123a08dc35-9825-4c17-aa01-4fe853f88928
名前 / ファイル | ライセンス | アクション |
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dsy021 (284.4 kB)
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Creative Commons
Attribution-NonCommercial 4.0 International (https://creativecommons.org/licenses/by-nc/4.0/) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-03-04 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Genome-wide characterization of DNA methylation, small RNA expression, and histone H3 lysine nine di-methylation in Brassica rapa L. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Takahashi, Satoshi
× Takahashi, Satoshi× Osabe, Kenji× Fukushima, Naoki× Takuno, Shohei× Miyaji, Naomi× Shimizu, Motoki× Takasaki-Yasuda, Takeshi× Suzuki, Yutaka× Dennis, Elizabeth S× Seki, Motoaki× Fujimoto, Ryo |
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書誌情報 |
en : DNA Research 巻 25, 号 5, p. 511-520, 発行日 2018-06-29 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Epigenetic gene regulation is crucial to plant life and can involve dynamic interactions between various histone modifications, DNA methylation, and small RNAs. Detailed analysis of epigenome information is anticipated to reveal how the DNA sequence of the genome is translated into the plant's phenotype. The aim of this study was to map the DNA methylation state at the whole genome level and to clarify the relationship between DNA methylation and transcription, small RNA expression, and histone H3 lysine 9 di-methylation (H3K9me2) in Brassica rapa. We performed whole genome bisulfite sequencing, small RNA sequencing, and chromatin immunoprecipitation sequencing using H3K9me2 antibody in a Chinese cabbage inbred line, RJKB-T24, and examined the impact of epigenetic states on transcription. Cytosine methylation in DNA was analysed in different sequence contexts (CG, CHG, and CHH) (where H could be A, C, or T) and position (promoter, exon, intron, terminator, interspersed repeat regions), and the H3K9me2 and 24 nucleotide small interfering RNAs (24 nt-siRNA) were overlaid onto the B. rapa reference genome. The epigenome was compared with that of Arabidopsis thaliana and the relationship between the position of DNA methylation and gene expression, and the involvement of 24 nt siRNAs and H3K9me2 are discussed. | |||||
出版者 | ||||||
出版者 | Oxford University Press on behalf of Kazusa DNA Research Institute | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1756-1663 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1340-2838 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/29982343 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1093/dnares/dsy021 | |||||
権利 | ||||||
権利情報 | ©2018 The Author(s). | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://academic.oup.com/dnaresearch/article/25/5/511/5047107#122670911 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |