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Nanoplasmonics for Real-Time and Label-Free Monitoring of Microbial Biofilm Formation
https://oist.repo.nii.ac.jp/records/852
https://oist.repo.nii.ac.jp/records/852950e9fef-a126-4888-9df7-ae32e95f3ea4
名前 / ファイル | ライセンス | アクション |
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Funari-2018-Nanoplasmonics for Real-Time and L (833.8 kB)
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ACS AuthorChoice
(https://pubs.acs.org/page/policy/authorchoice_termsofuse.html) |
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-05-08 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Nanoplasmonics for Real-Time and Label-Free Monitoring of Microbial Biofilm Formation | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者(英) |
Funari, Riccardo
× Funari, Riccardo× Bhalla, Nikhil× Chu, Kang-Yu× Söderström, Bill× Shen, Amy Q. |
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書誌情報 |
en : ACS Sensors 巻 3, 号 8, p. 1499-1509, 発行日 2018-07-31 |
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抄録 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Microbial biofilms possess intrinsic resistance against conventional antibiotics and cleaning procedures; thus, a better understanding of their complex biological structures is crucial in both medical and industrial applications. Existing laboratory methodologies have focused on macroscopic and mostly indirect characterization of mechanical and microbiological properties of biofilms adhered on a given substrate. However, the kinetics underlying the biofilm formation is not well understood, while such information is critical to understanding how drugs and chemicals influence the biofilm formation. Herein, we report the use of localized surface plasmon resonance (LSPR) for real-time, label-free monitoring of E. coli biofilm assembly on a nanoplasmonic substrate consisting of gold mushroom-like structures. Our LSPR sensor is able to capture the signatures of biofilm formation in real-time by measuring the wavelength shift in the LSPR resonance peak with high temporal resolution. We employ this sensor feature to elucidate how biofilm formation is affected by different drugs, including conventional antibiotics (kanamycin and ampicillin) as well as rifapentine, a molecule preventing cell adhesion yet barely affecting bacterial viability and vitality. Due to its flexibility and simplicity, our LSPR based platform can be used on a wide variety of clinically relevant bacteria, thus representing a valuable tool in biofilm characterization and drug screening. | |||||
出版者 | ||||||
出版者 | American Chemical Society | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2379-3694 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | info:pmid/30062880 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1021/acssensors.8b00287 | |||||
権利 | ||||||
権利情報 | © 2018 American Chemical Society | |||||
情報源 | ||||||
関連名称 | https://pubs.acs.org/page/policy/authorchoice_termsofuse.html | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://pubs.acs.org/doi/10.1021/acssensors.8b00287 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |